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Beta-Actin (ACTB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS191358

Actins are essential components of the cytoskeleton with critical roles in a wide range of cellular processes including cell migration, cell division, and the regulation of gene expression. Beta-actin, product of the Actb gene, is one of six different actin… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Beta-Actin (ACTB), Polyclonal Antibody
Full Product Name
Beta-Actin Polyclonal Antibody
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Form/Format
100ug (1mg/ml) peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Specificity
This antibody recognizes human beta-actin.
Preparation and Storage
This antibody is stable for at least one (1) year at-20 degree C.
Molecular Weight
41,737 Da
3D Structure
ModBase 3D Structure for P60709
Immunogen
Synthetic peptide corresponding to mid-protein amino acids of human beta-actin.
Applications Tested/Suitable for anti-ACTB antibody
Immunoblot, Immunofluorescence (IF)
Application Notes for anti-ACTB antibody
Immunoblot: 1ug/ml
IF: 10ug/ml
Dilute in PBS or medium that is identical to that used in the assay system.

Testing Data of anti-ACTB antibody

Detection of beta-actin in HeLa cell lysate (left) and 293T cell lysate (right) at 1ug/ml.
Rabbit Anti-ACTB Polyclonal Antibody – Cat# MBS191358

Testing Data of anti-ACTB antibody

Detection of beta-actin in HeLa cells at 10ug/ml. DAPI (blue) nuclear stain and FITC (green) beta-actin stain.
Rabbit Anti-ACTB Polyclonal Antibody, Testing Data – Cat# MBS191358

Other Notes

Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS191358 is an antibody targeting ACTB. Also known as Beta-Actin Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
UniProt Gene Name
ACTB
NCBI Protein Information
actin, cytoplasmic 1

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
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UniProt Comments for ACTB

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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