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Brain-Derived Neurotrophic Factor (rHuBDNF) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS191481

Recombinant Human BDNF produced in E Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 119 amino acids with a molecular mass of 26,984 daltons. This product has full biological activity. More details

$280 / 0.002 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Brain-derived Neurotrophic Factor (rHuBDNF), Active Protein
Full Product Name
Recombinant Human Brain-derived Neurotrophic Factor (rHuBDNF)
Product Gene Name
rHuBDNF active protein
Product Synonym Names
Human Brain-derived Neurotrophic Factor (rHuBDNF)
Host
E Coli
Form/Format
Sterile filtered, lyophilized.
Purity/Purification
Greater than 96% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Preparation and Storage
Lyophilized product is stable at room temperature for 3 weeks.
Store lyophilized product desiccated at-20 degree C.
Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at-20 degree C.
For long-term storage add either HSA or BSA to 0.1%.
Avoid repeated freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Fully biologically active compared to reference standards. ED50 in rat basal forebrain primary septal culture is 50ng/ml.
Endotoxin
<0.1 ng/ug
Stabilizers
None
Preservatives
None
Protein Content
Determined spectrophotometrically at 280nm (1.6 ext. coeff), and by RP-HPLC analysis against a calibrated standard.
Dimers/Aggregates
<1% by SDS-PAGE
Reconstitution
Reconstitute the lyophilized product in sterile water not less than 100 ug/ml. Product was dialyzed against 20mM sodium citrate pH 5 prior to lyophilization.

Other Notes

Small volumes of rHuBDNF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rHuBDNF purchase

MBS191481 is an active protein targeting rHuBDNF. Also known as Recombinant Human Brain-derived Neurotrophic Factor (rHuBDNF). Host: E Coli.
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