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Macrophage Inflammatory Protein-1 Alpha (Rhumip-1 Alpha) (CCL3) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS197017

Recombinant Human MIP-1 alpha produced in E. coli is a single, nonglycosylated, polypeptide chain containing 70 amino acids with a molecular mass of 7,820 daltons. This product has full biological activity. More details

$265 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha) (CCL3), Active Protein
Full Product Name
Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)
Product Gene Name
CCL3 active protein
Product Synonym Names
Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)
Host
E Coli
Form/Format
Sterile filtered, lyophilized.
Purity/Purification
Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Purification: Chromatographic
Specificity
Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)
Preparation and Storage
Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20 degree C. Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at -20 degree C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.
Molecular Weight
10,085 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (34415602..34417506, complement). Location: 17q12
OMIM
182283
3D Structure
ModBase 3D Structure for P10147
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Dimers/Aggregates
<1% by SDSPAGE
Biological Activity
Fully biologically active compared to reference standards. Activity determined by the ability to chemoattract human monocytes at a concentration of 1-10 ng/ml.
Endotoxin Level
<0.1 ng/ug
Protein Content
Determined spectrophotometrically at 280nm (extinction coefficient 0.9) and by RPHPLC analysis against a calibrated standard.
Solubility
Reconstitute the lyophilized product in sterile water not less than 100 ug/ml
Stabilizer
None
Preservatives
None

Product Categories/Family for CCL3 active protein

Recombinant Proteins

Other Notes

Small volumes of CCL3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL3 purchase

MBS197017 is an active protein targeting CCL3. Also known as Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL3. It may not necessarily be applicable to this product.

NCBI GI #
2905628
NCBI GeneID
6348
NCBI Accession #
AAC03539.1
UniProt Primary Accession #
P10147
UniProt Related Accession #
P10147; Q14745
NCBI Official Full Name
macrophage inflammatory protein 1 alpha
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
CCL3
NCBI Official Synonym Symbols
MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
UniProt Protein Name
C-C motif chemokine 3
UniProt Synonym Protein Names
G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinCleaved into the following chain:MIP-1-alpha(4-69); Alternative name(s):; LD78-alpha(4-69)
Protein Family
C-C motif chemokine
UniProt Gene Name
CCL3
UniProt Synonym Gene Names
G0S19-1; MIP1A; SCYA3
UniProt Entry Name
CCL3_HUMAN
NCBI Protein Information
C-C motif chemokine 3; SIS-beta; PAT 464.1; OTTHUMP00000163953; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a)

NCBI Summary for CCL3

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.
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UniProt Comments for CCL3

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Chemokine; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol

Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; chemoattractant activity; CCR5 chemokine receptor binding; phospholipase activator activity; protein kinase activity

Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; regulation of cell shape; negative regulation of bone mineralization; monocyte chemotaxis; cell-cell signaling; positive chemotaxis; calcium ion transport; positive regulation of neuron apoptosis; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of cell migration; positive regulation of inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
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Research Articles on CCL3

1. MIP-1alpha induces proliferation of nonasthmatic airway smooth muscle cells and decreases their rate of apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CCL3 active protein

 Reference Product  PubMed Publications
 CCR5 active protein  >418 publications with CCL3 and CCR5
 CCR1 active protein  >232 publications with CCL3 and CCR1
 CCR3 active protein  >60 publications with CCL3 and CCR3
 CCR4 active protein  >36 publications with CCL3 and CCR4
 TGFB1 active protein  >28 publications with CCL3 and TGFB1
 BCL6 active protein  >3 publications with CCL3 and BCL6
 CEBPB active protein  >1 publications with CCL3 and CEBPB

Pathways associated with CCL3 active protein

 Products by Pathway  Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram

Diseases associated with CCL3 active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >618 publications with CCL3 and Inflammation
 Nervous System Diseases Antibodies  >194 publications with CCL3 and Nervous System Diseases
 Lung Diseases Antibodies  >181 publications with CCL3 and Lung Diseases
 HIV Infections Antibodies  >161 publications with CCL3 and HIV Infections
 Cardiovascular Diseases Antibodies  >123 publications with CCL3 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >114 publications with CCL3 and Vascular Diseases
 Skin Diseases Antibodies  >75 publications with CCL3 and Skin Diseases
 Leukemia Antibodies  >73 publications with CCL3 and Leukemia
 Arthritis, Rheumatoid Antibodies  >69 publications with CCL3 and Arthritis, Rheumatoid
 Liver Diseases Antibodies  >65 publications with CCL3 and Liver Diseases

Organs/Tissues associated with CCL3 active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1186 publications with CCL3 and Blood
 Lung Antibodies  >245 publications with CCL3 and Lung
 Brain Antibodies  >179 publications with CCL3 and Brain
 Liver Antibodies  >159 publications with CCL3 and Liver
 Kidney Antibodies  >60 publications with CCL3 and Kidney
 Heart Antibodies  >56 publications with CCL3 and Heart
 Eye Antibodies  >39 publications with CCL3 and Eye
 Intestine Antibodies  >39 publications with CCL3 and Intestine
 Uterus Antibodies  >17 publications with CCL3 and Uterus
 Placenta Antibodies  >14 publications with CCL3 and Placenta
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