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CCL14 (HCC-1) (CCL14) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS191501

CCL14, also known as Hemofiltrate CC Chemokine-1 (HCC-1) is a small cytokine (~8kDa) that is constitutively expressed in multiple tissues. Upon processing of the N-terminal residues of fulllength HCC-1 by the uPA-plasmin system, the active form of HCC-1 is a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CCL14 (HCC-1), Active Protein
Full Product Name
Recombinant CCL14 (HCC-1), biotinylated
Product Gene Name
Product Synonym Names
Human CCL14, biotinylated
Sequence
GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVC TNPSDKWVQD YIKDMKEN
Host
E Coli
Form/Format
Lyophilized
Purity/Purification
>97% by SDS-PAGE
Preparation and Storage
12 months from date of receipt,-20 degree C to-70 degree C, as supplied.
1 month,-20 degree C to-70 degree C, under sterile conditions after reconstitution.
Best if used immediately after reconstitution.
Molecular Weight
10.22kDa by Mass Spec.
3D Structure
ModBase 3D Structure for Q16627
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source
Human
Endotoxin
<0.01 EU per 1ug of protein by LAL method.
Activity
EC50 = 0.2-0.4nM determined by Migration Assay in cells expressing CCR1.
Modification
Biotinylated
Reconstitution
Recommended at 100ug/ml in sterile distilled water.
Carrier Protein
None

Other Notes

Small volumes of CCL14 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL14 purchase

MBS191501 is an active protein targeting CCL14. Also known as Recombinant CCL14 (HCC-1), biotinylated. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL14. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
C-C motif chemokine 14 isoform 2
NCBI Official Synonym Full Names
C-C motif chemokine ligand 14
NCBI Official Symbol
CCL14
NCBI Official Synonym Symbols
CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
UniProt Protein Name
C-C motif chemokine 14
UniProt Synonym Protein Names
Chemokine CC-1/CC-3; HCC-1/HCC-3
Protein Family
UniProt Gene Name
CCL14
UniProt Synonym Gene Names
NCC2; SCYA14; HCC-1/HCC-3
NCBI Protein Information
C-C motif chemokine 14

NCBI Summary for CCL14

This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]
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UniProt Comments for CCL14

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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