PD-1 Antibody – Mouse Monoclonal

Immunohistochemistry: IgA control JC053
CHO-PD1 cells fixed with 10% neutral buffered formalin, prepared in Histogel and embedded in paraffin, antigen retrieval in citrate buffer at pH6. Cells stained with IgA isotype control or with JC053.
Tonsil, IgA control Tonsil, JC053 LympHnode, IgA control LympHnode, JC053
Formalin-fixed, paraffin-embedded canine tonsil and lympHnode stained with IgA isotype control or with JC053.
Flow Cytometry: Detection of PD-1 expression on CLGL-90 cells (Chronic Large Granular Lymphocytic-90) stained with JC053 (dark gray) vs. mouse IgA isotype control (light gray).
Inhibition of PD-1 and PD-L1 Interaction
JC053 antibody added to ELISA plates coated with PD-1 followed by the addition of biotinylated PD-L1and streptavidin-HRP. JC053 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of a non-inhibitory antibody 73H2.
Endusers should determine optimal antibody concentrations for their applications.