COVID 19 IgG Coronavirus (COVID-19) Assay Kit

Highly purified recombinant antigens (NP, RBD and S1 for SARS-CoV-2, as well as NP for NL63, OC43, 229E, HKU1) are fixed to nitrocellu-lose membrane test strips. 1. The test strips are incubated with the diluted serum or plasma sample with specific antibodies binding to the pathogen antigens on the test strips. 2. Unbound antibodies are then washed away. 3. The strips are incubated in a second step with anti-human immu-noglobulin antibodies (IgG) that are coupled to horseradish peroxi-dase. 4. Unbound conjugate antibodies are then washed away. 5. Specific bound antibody is detected in a colour reaction catalysed by the peroxidase. If an antigen-antibody reaction has taken place, a dark band appears on the strip at the corresponding location.
Control bands are located at the top end of the test strip:
a) The reaction control below the strip number for which every se-rum/plasma sample must show a reaction.
b) The conjugate controls (IgG) are used to check the conjugate and strip type used (Ig class specific).
c) 'Cut-off control': The intensity of this band enables an evaluation of the reactivity of the individual antigen bands (see 9.2 Analysis).

Infectious Disease Test Kits and Positive Controls; MIKROGEN Products