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Agarose Immobilized Goat Anti-6-His Antibody – Goat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS194005

A polystidine-tag consists of at least six histidine (His) residues, often at the N-or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Agarose Immobilized Goat anti-6-his, Antibody
Full Product Name
6-His Affinity Purified Antibody, Agarose Immobilized
Product Synonym Names
Agarose Immobilized Goat anti-6-his
Host
Goat
Form/Format
0.1mg/0.4ml 50% gel slurry in Phosphate-Buffered Saline (PBS), pH 7.2, 0.09% sodium azide. Antibody coupled to agarose beads using cyanogen bromide.
Concentration
100 ug antibody / 200 ul gel (lot specific)
Specificity
Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Preparation and Storage
This antibody is stable for at least one year at 2-8 degree C.
Antigen
6-His (HHHHHH) coupled to KLH. Antibody coupled to agarose beads using a cyanogen bromide method.
Buffer
0.01M Phosphate, pH 7.2, 0.1M NaCl
Stabilizer
None
Preservatives
0.1% sodium azide

Product Categories/Family for anti-Agarose Immobilized Goat anti-6-his antibody

Epitope Tag Antibodies
Applications Tested/Suitable for anti-Agarose Immobilized Goat anti-6-his antibody
Immunoprecipitation
Application Notes for anti-Agarose Immobilized Goat anti-6-his antibody
Immunoprecipitation: 15 to 25 ul of gell slurry per 0.1 to 1 mg of protein lysate or extract.

Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system.

Other Notes

Small volumes of anti-Agarose Immobilized Goat anti-6-his antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Agarose Immobilized Goat anti-6-his purchase

MBS194005 is an antibody targeting Agarose Immobilized Goat anti-6-his. Also known as 6-His Affinity Purified Antibody, Agarose Immobilized. Host: Goat.
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