PYK2 Antibody – Rabbit

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); Nuclear receptor co-regulator; TK group; Fak family

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: focal adhesion; dendrite; postsynaptic density; cell cortex; cytosol; N-methyl-D-aspartate selective glutamate receptor complex; lipid raft; nucleoplasm; extrinsic to internal side of plasma membrane; growth cone; cytoskeleton; cell soma; perinuclear region of cytoplasm; axon; lamellipodium; cytoplasm; nucleus

Molecular Function: signal transducer activity; protein binding; calmodulin-dependent protein kinase activity; protein-tyrosine kinase activity; 3-phosphoinositide-dependent protein kinase binding; N-methyl-D-aspartate selective glutamate receptor activity; non-membrane spanning protein tyrosine kinase activity; protein complex binding; ATP binding; receptor binding

Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; glial cell proliferation; negative regulation of bone mineralization; regulation of inositol trisphosphate biosynthetic process; regulation of cell shape; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; response to glucose stimulus; protein complex assembly; negative regulation of neuron apoptosis; neurite development; bone resorption; response to drug; negative regulation of myeloid cell differentiation; positive regulation of nitric-oxide synthase activity; response to osmotic stress; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to calcium ion; negative regulation of apoptosis; activation of JAK protein; peptidyl-tyrosine phosphorylation; response to cAMP; positive regulation of translation; blood vessel endothelial cell migration; apoptosis; protein amino acid autophosphorylation; response to lithium ion; negative regulation of potassium ion transport; response to hormone stimulus; regulation of calcium-mediated signaling; positive regulation of JNK cascade; signal transduction; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; response to stress; angiogenesis; positive regulation of cell-matrix adhesion; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; MAPKKK cascade; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; response to cocaine; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; response to hydrogen peroxide; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; stress fiber formation; response to hypoxia; cellular defense response; innate immune response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration