Recombinant Human Thyroid Peroxidase (TPO) Recombinant Protein

Thyroid peroxidase (TPO) is an integral apical membrane glycoprotein of thyroid follicular cells and plays a key role in thyroid hormone biosynthesis. It is a homodimer comprised of two identical subunits of approx. 100kDa. TPO is one of the main autoantigenic targets in autoimmune thyroid disease of humans. Anti-TPO autoantibodies are present in 90% of cases of Hashimoto's thyroiditis and 75% of cases of Graves' disease.

Recombinant human TPO produced in insect cells corresponds to aa 19-846 (calculated molecular weight approx. 92kDa) of the soluble extracellular domain of human TPO. It does not contain any tags. It is purified by specific monoclonal antibody-based affinityand ion exchange chromato-graphic methods. In SDS-PAGE, two bands in the region of 100-110kDa are observed due to differential glycosyl- ation of the protein. Immunoreactivity of rhuTPO is confirmed by ELISA with patient serum samples containing anti-TPO autoantibodies.

Small volumes of Recombinant Human Thyroid Peroxidase (TPO) recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS197032 is a recombinant protein targeting Recombinant Human Thyroid Peroxidase (TPO). Host: Insect Cell.