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Fibrinogen (FBG) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS019790

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of FBG (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibrinogen (FBG), ELISA Kit
Full Product Name
Mouse Fibrinogen ELISA Kit
Product Gene Name
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Detection Range
0.25mg/ml-8mg/ml
Sensitivity
0.1mg/ml
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
Intra-assay Precision
Intra-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Molecular Weight
50,244 Da
Chromosome Location
Chromosome: 17; AC_000174.1 (2823044..2830515). Location: 17q12-q13
3D Structure
ModBase 3D Structure for P12799

Other Notes

Small volumes of FBG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FBG purchase

MBS019790 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibrinogen (FBG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FBG. The ELISA analytical biochemical technique of the MBS019790 kit is based on FBG antibody-FBG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FBG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FBG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FBG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
fibrinogen
NCBI Official Synonym Full Names
fibrinogen gamma chain
NCBI Official Symbol
FGG
UniProt Protein Name
Fibrinogen gamma-B chain
Protein Family
UniProt Gene Name
FGG
UniProt Synonym Gene Names
Gamma'
UniProt Entry Name
FIBG_BOVIN
NCBI Protein Information
fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide

UniProt Comments for FBG

Function: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Subunit structure: Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain

By similarity.

Subcellular location: Secreted.

Domain: A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

Post-translational modification: Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.

Sequence similarities: Contains 1 fibrinogen C-terminal domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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