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A Crystallin A (CRYAA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS200007

alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced by heat shock… More details

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Product Overview

Online Datasheet in PDF
Product Name
A crystallin A (CRYAA), Monoclonal Antibody
Full Product Name
A crystallin A antibody
Product Gene Name
anti-CRYAA antibody
Product Synonym Names
Heat shock protein beta-4; HspB4; Alpha-crystallin A chain; Heat shock protein beta 4; Acry-1; Alpha crystallin A chain; CRYA 1; CRYA1; CRYAA; Crystallin Alpha 1; Crystallin alpha A; HSPB 4; Zonular Central Nuclear Cataract
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
c9F2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at 2°C to 8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
OMIM
U05569 mRNA
3D Structure
ModBase 3D Structure for P02489
Immunogen
Recombinant human Crystallin alpha A (1-173aa) purified from E. coli

Product Categories/Family for anti-CRYAA antibody

Heat Shock Proteins
Applications Tested/Suitable for anti-CRYAA antibody
ELISA (EIA), Western Blot (WB), FACS
Application Notes for anti-CRYAA antibody
Since applications varies, however, each investigation should be titrated by reagent to obtain optimal results.

Western Blot (WB) of anti-CRYAA antibody

The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
This antibody is not shown cross-activity about Crystallin alpha B.
Lane 1.: Recombinant human Crystallin alpha A protein
Lane 2.: Recombinant human Crystallin alpha B protein
Mouse Anti-CRYAA Monoclonal Antibody – Cat# MBS200007

Western Blot (WB) of anti-CRYAA antibody

The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Crystallin alpha A Transfected 293T cell lysate
Mouse Anti-CRYAA Monoclonal Antibody, Western Blot (WB) – Cat# MBS200007

Flow Cytommetry (FC) of anti-CRYAA antibody

Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cells. The cell was stained with MBS200007 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-CRYAA Monoclonal Antibody, Flow Cytommetry (FC) – Cat# MBS200007

Product References and Citations for anti-CRYAA antibody

Fujii N., et al., (2003) Mol. Vis. 9: 915-22; Laksanalamai P., et al., (2004) Extremophiles 8: 1-11

Other Notes

Small volumes of anti-CRYAA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRYAA purchase

MBS200007 is an antibody targeting CRYAA. Also known as A crystallin A antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CRYAA. It may not necessarily be applicable to this product.

NCBI GI #
4503055
NCBI GeneID
1409
NCBI Accession #
NP_000385
UniProt Primary Accession #
P02489
UniProt Secondary Accession #
Q53X53
UniProt Related Accession #
P02489
GenBank Nucleotide #
NM_000394.3
NCBI Official Full Name
alpha-crystallin A chain
NCBI Official Synonym Full Names
crystallin alpha A
NCBI Official Symbol
CRYAA
NCBI Official Synonym Symbols
CRYA1; HSPB4; CTRCT9
UniProt Protein Name
Alpha-crystallin A chain
UniProt Synonym Protein Names
Heat shock protein beta-4; HspB4
Protein Family
Alpha-crystallin
UniProt Gene Name
CRYAA
UniProt Synonym Gene Names
CRYA1; HSPB4; HspB4
NCBI Protein Information
alpha-crystallin A chain

NCBI Summary for CRYAA

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]
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UniProt Comments for CRYAA

Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.

Research Articles on CRYAA

1. The results suggest that the N-terminal domain of CRYAA is required during in vitro complex formation with filensin and phakinin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CRYAA antibody

 Reference Product  PubMed Publications
 CRYAB antibody  >35 publications with CRYAA and CRYAB
 CRYGC antibody  >28 publications with CRYAA and CRYGC
 CRYBB2 antibody  >27 publications with CRYAA and CRYBB2
 GJA8 antibody  >25 publications with CRYAA and GJA8
 CRYBB1 antibody  >20 publications with CRYAA and CRYBB1
 CRYBA1 antibody  >17 publications with CRYAA and CRYBA1
 CRYGS antibody  >13 publications with CRYAA and CRYGS
 MIP antibody  >11 publications with CRYAA and MIP
 CRYBB3 antibody  >10 publications with CRYAA and CRYBB3
 CRYGA antibody  >9 publications with CRYAA and CRYGA

Pathways associated with anti-CRYAA antibody

 Products by Pathway  Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram

Organs/Tissues associated with anti-CRYAA antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >91 publications with CRYAA and Eye
 Brain Antibodies  >4 publications with CRYAA and Brain
 Liver Antibodies  >1 publications with CRYAA and Liver
 Thymus Antibodies  >1 publications with CRYAA and Thymus
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