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IRF-5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200023

Interferon regulatory factor 5 (IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IRF-5, Monoclonal Antibody
Full Product Name
IRF-5 antibody
Product Gene Name
Product Synonym Names
interferon regulatory factor 5; irf5
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
10T1
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
17,020 Da
OMIM
AY504947 mRNA
Antigen Species
Human
Immunogen
Recombinant human IRF-5 (176-240aa) purified from E. coli
Clone Note
Anti-human IRF-5 mAb, clone 10T1, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IRF-5 protein.

Product Categories/Family for anti-IRF-5 antibody

Applications Tested/Suitable for anti-IRF-5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-IRF-5 antibody
The antibody has been tested by ELISA, Western blot, IP, and immunohistochemical analysis to assure specificity and reactivity.
WB: 1:500 ~ 1:1,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-IRF-5 antibody

The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-IRF-5 Monoclonal Antibody – Cat# MBS200023

Immunohistochemistry (IHC) of anti-IRF-5 antibody

Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Mouse Anti-IRF-5 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200023

Immunofluorescence (IF) of anti-IRF-5 antibody

ICC/IF analysis of IRF5 in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human THP-1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of IRF5 in Raw264.7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IRF5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-IRF-5 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200023

Product References and Citations for anti-IRF-5 antibody

Mario B. et al. (2007) J. Virology 81 (9):4473-4481.; Ning S. et al. (2005) J. Virology 79:11671-11676.; Mario B. et al. (2007) J. Virology 81 (9):4473-4481

Other Notes

Small volumes of anti-IRF-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRF-5 purchase

MBS200023 is an antibody targeting IRF-5. Also known as IRF-5 antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IRF-5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Synonym Full Names
interferon regulatory factor 5
NCBI Official Symbol
IRF5
NCBI Official Synonym Symbols
SLEB10
UniProt Protein Name
Interferon regulatory factor 5
UniProt Gene Name
IRF5
UniProt Synonym Gene Names
IRF-5
NCBI Protein Information
interferon regulatory factor 5

NCBI Summary for IRF-5

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]
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UniProt Comments for IRF-5

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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