• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Geminin (GMNN) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200034

Geminin is a 25 kDa protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, geminin… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Geminin (GMNN), Monoclonal Antibody
Full Product Name
Geminin antibody
Product Gene Name
Product Synonym Names
GMNN; Geminin; Geminin; DNA replication inhibitor; Gem; Geminin DNA replication inhibitor; OTTHuMP00000039393; RP3 369A17.3.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
2H7
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23,565 Da
OMIM
AF067855 mRNA
3D Structure
ModBase 3D Structure for O75496
Antigen Species
Human
Immunogen
Recombinant human Geminin (1-209 aa) purified from E. coli
Clone Note
Anti-human Geminin mAb, clone 2H7, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Geminin protein.

Product Categories/Family for anti-GMNN antibody

Applications Tested/Suitable for anti-GMNN antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-GMNN antibody
WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.

Western Blot (WB) of anti-GMNN antibody

The K562 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Geminin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-GMNN Monoclonal Antibody – Cat# MBS200034

Product References and Citations for anti-GMNN antibody

McGarry TJ. et al., (1998) Cell. 93 (6):1043-53.; Pitulescu M. et al., (2005) Cell Mol Life Sci. 62 (13):1425-33.; Luo L. et al., (2004) Nature. 427 (6976):749-53

Other Notes

Small volumes of anti-GMNN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GMNN purchase

MBS200034 is an antibody targeting GMNN. Also known as Geminin antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GMNN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
geminin
NCBI Official Synonym Full Names
geminin, DNA replication inhibitor
NCBI Official Symbol
GMNN
NCBI Official Synonym Symbols
Gem; MGORS6
UniProt Protein Name
Geminin
Protein Family
UniProt Gene Name
GMNN
NCBI Protein Information
geminin

NCBI Summary for GMNN

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]
Show more

UniProt Comments for GMNN

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000