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TRA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200040

Tumor rejection antigen 1 (TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells. More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRA1, Monoclonal Antibody
Full Product Name
TRA1 antibody
Product Gene Name
Product Synonym Names
94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; HSP90B1; GP96; TRA1; Tumor rejection antigen 1; GRP94; 94 kDa glucose-regulated protein 94 kDa glucose regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 9; Heat shock protein 90 kDa beta member 1; Stress inducible tumor rejection antigen GP96; Tumor rejection antigen gp96.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
2H3
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
92,469 Da
OMIM
X15187 mRNA
3D Structure
ModBase 3D Structure for P14625
Antigen Species
Human
Immunogen
Recombinant human TRA1 (676-803aa) purified from E. coli
Clone Note
Anti-human TRA1 mAb, clone 2H3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TRA1 protein.

Product Categories/Family for anti-TRA1 antibody

Applications Tested/Suitable for anti-TRA1 antibody
ELISA (EIA), WB (Cell lysate), Immunofluorescence (IF)
Application Notes for anti-TRA1 antibody
IF: 1:100 ~ 1:200.
Recommended starting dilution is 1:100.

Western Blot (WB) of anti-TRA1 antibody

The cell lysates of hES (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA1 (1:500). Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : ES cell lysate
Mouse Anti-TRA1 Monoclonal Antibody – Cat# MBS200040

Immunofluorescence (IF) of anti-TRA1 antibody

Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)
Mouse Anti-TRA1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200040

Product References and Citations for anti-TRA1 antibody

Wulf GM, et al., (2002) J Biol. Chem. 277 (50)47976-47979; Hamdane M, et al., (2002) J Mol Neurosci. 19 (3) ; 275-287 Zheng H, et al., (2002) Nature 419 (6909) 853-857; Lu,k.P. et al., (1996) Nature 380 (6574) 544-547.; Campbell,H.D, et al., (1997) Genomics 44 (2), 157-162

Other Notes

Small volumes of anti-TRA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRA1 purchase

MBS200040 is an antibody targeting TRA1. Also known as TRA1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TRA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90 beta family member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
UniProt Protein Name
Endoplasmin
UniProt Synonym Protein Names
94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94
NCBI Protein Information
endoplasmin

NCBI Summary for TRA1

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
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UniProt Comments for TRA1

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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