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HSP27 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200046

Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress… More details

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Product Overview

Online Datasheet in PDF
Product Name
Hsp27, Monoclonal Antibody
Full Product Name
Hsp27 antibody
Product Gene Name
anti-Hsp27 antibody
Product Synonym Names
Heat shock 27 kDa protein; Hspb1; Hsp25; Hsp27; Hsp27; HspB1; Heat shock 27 kDa protein; HSP 27; Growth-related 25 kDa protein; P25; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; at shock 25kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat Shock Protein27; HS.76067; Hsp 28; Hsp B1; Hsp28; HspB1; SRP27; Stress responsive protein 27.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
2A5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
22,783 Da
OMIM
L39370 Genomic DNA
3D Structure
ModBase 3D Structure for P04792
Antigen Species
Human
Immunogen
Recombinant human Hsp27 (1-205aa) purified from E. coli
Clone Note
Anti-human Hsp27 mAb, clone 2A5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp27 protein.

Product Categories/Family for anti-Hsp27 antibody

Heat Shock Proteins
Applications Tested/Suitable for anti-Hsp27 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Hsp27 antibody
Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

Western Blot (WB) of anti-Hsp27 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: MCF7 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A431 cell lysate Lane 2.: HepG2 cell lysate
Mouse Anti-HSP27 Monoclonal Antibody – Cat# MBS200046

Immunohistochemistry (IHC) of anti-Hsp27 antibody

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Mouse Anti-HSP27 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200046

Immunofluorescence (IF) of anti-Hsp27 antibody

ICC/IF analysis of Hsp27 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Hsp27 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-HSP27 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200046

Product References and Citations for anti-Hsp27 antibody

Ehrnsperger M, et al., (1997) EMBO J 16:221-229; Steve J, et al., (2000) Mol Cell Biol 20 (20):7602-7612; Butt E, et al., (2001) J Biol Chem 276:7108-7113; Michael, et al., (2002) Cell Stress Chsperones 7 (3):281-296

Other Notes

Small volumes of anti-Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hsp27 purchase

MBS200046 is an antibody targeting Hsp27. Also known as Hsp27 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.

NCBI GI #
4504517
NCBI GeneID
3315
NCBI Accession #
NP_001531
UniProt Primary Accession #
P04792
UniProt Secondary Accession #
Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
UniProt Related Accession #
P04792
GenBank Nucleotide #
NM_001540.4
NCBI Official Full Name
heat shock protein beta-1
NCBI Official Synonym Full Names
heat shock protein family B (small) member 1
NCBI Official Symbol
HSPB1
NCBI Official Synonym Symbols
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
UniProt Protein Name
Heat shock protein beta-1
UniProt Synonym Protein Names
28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Protein Family
Heat shock protein
UniProt Gene Name
HSPB1
UniProt Synonym Gene Names
HSP27; HSP28; HspB1; HSP 27; SRP27
NCBI Protein Information
heat shock protein beta-1

NCBI Summary for Hsp27

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
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UniProt Comments for Hsp27

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
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Research Articles on Hsp27

1. found knock down of HSPB1 further increased the proportion of apoptotic cells in hyperthermic treated melanoma cells when compared with either single agent alone, and both agents leaded to cell cycle arrest at G0/G1 or G2/M phases

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hsp27 antibody

 Reference Product  PubMed Publications
 MAPKAPK2 antibody  >59 publications with Hsp27 and MAPKAPK2
 HSPA1A antibody  >29 publications with Hsp27 and HSPA1A
 HSPA8 antibody  >22 publications with Hsp27 and HSPA8
 MAPK14 antibody  >7 publications with Hsp27 and MAPK14
 MAPKAPK5 antibody  >5 publications with Hsp27 and MAPKAPK5
 MAPKAPK3 antibody  >4 publications with Hsp27 and MAPKAPK3
 ESR1 antibody  >2 publications with Hsp27 and ESR1
 EIF4G1 antibody  >1 publications with Hsp27 and EIF4G1

Pathways associated with anti-Hsp27 antibody

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 ATM Signaling Network In Development And Disease Pathway antibodies  ATM Signaling Network In Development And Disease Pathway Diagram
 AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies  Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation antibodies  Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram

Diseases associated with anti-Hsp27 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >114 publications with Hsp27 and Disease Models, Animal
 Inflammation Antibodies  >85 publications with Hsp27 and Inflammation
 Necrosis Antibodies  >83 publications with Hsp27 and Necrosis
 Heart Diseases Antibodies  >66 publications with Hsp27 and Heart Diseases
 Liver Diseases Antibodies  >51 publications with Hsp27 and Liver Diseases
 Lung Neoplasms Antibodies  >43 publications with Hsp27 and Lung Neoplasms
 Reperfusion Injury Antibodies  >42 publications with Hsp27 and Reperfusion Injury
 Kidney Diseases Antibodies  >38 publications with Hsp27 and Kidney Diseases
 Liver Neoplasms Antibodies  >33 publications with Hsp27 and Liver Neoplasms
 Carcinoma, Squamous Cell Antibodies  >32 publications with Hsp27 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-Hsp27 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >263 publications with Hsp27 and Muscle
 Blood Antibodies  >207 publications with Hsp27 and Blood
 Brain Antibodies  >199 publications with Hsp27 and Brain
 Heart Antibodies  >134 publications with Hsp27 and Heart
 Nerve Antibodies  >124 publications with Hsp27 and Nerve
 Vascular Antibodies  >115 publications with Hsp27 and Vascular
 Lung Antibodies  >95 publications with Hsp27 and Lung
 Liver Antibodies  >94 publications with Hsp27 and Liver
 Kidney Antibodies  >81 publications with Hsp27 and Kidney
 Eye Antibodies  >56 publications with Hsp27 and Eye
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