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BHMT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200050

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from… More details

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Product Overview

Online Datasheet in PDF
Product Name
BHMT, Monoclonal Antibody
Full Product Name
BHMT antibody
Product Gene Name
Product Synonym Names
Betaine-homocysteine methyltransferase; BHMT; BHMT (Betaine-homocysteine methyltransferase); EC 2.1.1.5; Betaine--homocysteine S-methyltransferase 1; Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase; Betaine homocysteine S methyltransferase; Betaine homocysteine S methyltransferase 1; BHMT1
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
3D6
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
44,998 Da
OMIM
U50929 mRNA
Antigen Species
Human
Immunogen
Recombinant human BHMT (1-406aa) purified from E. coli
Clone Note
Anti-human BHMT mAb, clone 3D6, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BHMT protein.

Product Categories/Family for anti-BHMT antibody

Applications Tested/Suitable for anti-BHMT antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-BHMT antibody
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-BHMT antibody

The extracts of mouse liver (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-BHMT Monoclonal Antibody – Cat# MBS200050

Immunofluorescence (IF) of anti-BHMT antibody

ICC/IF analysis of BHMT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BHMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-BHMT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200050

Product References and Citations for anti-BHMT antibody

Mckeever MP et al., (1991) Clin Sci 81 (4):551-556; Norihiko F et al., (2001) Biochem J 129:313-320; Francisco G et al., (2005) Biochem J 391:589-599; Catherine M et al., (2005) Biochem J 392:443-448; Janet D. S et al., (2006) Biochem J 395:363-371

Other Notes

Small volumes of anti-BHMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BHMT purchase

MBS200050 is an antibody targeting BHMT. Also known as BHMT antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BHMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Betaine-homocysteine methyltransferase
NCBI Official Synonym Full Names
betaine--homocysteine S-methyltransferase
NCBI Official Symbol
BHMT
NCBI Official Synonym Symbols
BHMT1; HEL-S-61p
UniProt Protein Name
Betaine--homocysteine S-methyltransferase 1
UniProt Gene Name
BHMT
NCBI Protein Information
betaine--homocysteine S-methyltransferase 1

NCBI Summary for BHMT

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

UniProt Comments for BHMT

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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