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UNG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200056

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of… More details

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Product Overview

Online Datasheet in PDF
Product Name
UNG, Monoclonal Antibody
Full Product Name
UNG antibody
Product Gene Name
anti-UNG antibody
Product Synonym Names
uracil-DNA glycosylase isoform UNG2; uracil-DNA glycosylase; UDG; UNG1; UNG2; HIGM4; uracil-DNA glycosylase 1; uracil-DNA glycosylase 2
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
k1C12
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
33,924 Da
OMIM
X15653 mRNA
3D Structure
ModBase 3D Structure for P13051
Antigen Species
Human
Immunogen
Recombinant human UNG (1-313aa) purified from E. coli
Clone Note
Anti-human UNG mAb, clone k1C12, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UNG protein.

Product Categories/Family for anti-UNG antibody

Hormones & Cytokines
Applications Tested/Suitable for anti-UNG antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-UNG antibody
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-UNG antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : K562 cell lysate Lane 5 : 293T cell lysate Lane 6 : HepG2 cell lysate Lane 7 : A549 cell lysate Lane 8 : SK-OV-3 cell lysate Lane 9 : PC3 cell lysate
Mouse Anti-UNG Monoclonal Antibody – Cat# MBS200056

Immunofluorescence (IF) of anti-UNG antibody

ICC/IF analysis of UNG in HeLa cells. The cell was stained with AUN0713 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-UNG Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200056

Product References and Citations for anti-UNG antibody

Alkylation Sensitivity Screens Reveal a Conserved Cross-species Functionome. David Svilar, Madhu Dyavaiah, Ashley R. Brown, Jiang-bo Tang, Jianfeng Li, Peter R. McDonald, Tong Ying Shun, Andrea Braganza, Xiao-hong Wang, Salony Maniar, Claudette M. St Croix, John S. Lazo, Ian F. Pollack, Thomas J. Begley, and Robert W. Sobol (2012)

Other Notes

Small volumes of anti-UNG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UNG purchase

DNA base excision repair is a current "in vogue" topic in the fields of oncology and molecular biology. The UNG gene product - uracil DNA glycosylase - is actively involved in this process and many researchers are engaged in finding families drug compounds that are not toxic but act as compounds expression function inhibitors for the human protein. The ability to localize the 2 forms of proteins by IHC or custom detections using IgG Alexa fluorâ® is possible with a UNG antibody (mouse monoclonal) found in products category at MyBiosource. This UNG mouse monoclonal antibody clone, purified with Protein G, reacts equally well with secondary antibodies anti mouse rabbit IgG or donkey anti / goat anti mouse in western blot as verified with transient overexpression lysate. To increase the value of this anti UNG antibody, the lysate in available as a free sample for use as a positive control.

Additional Details

NCBI/Uniprot data below describe general gene information for UNG. It may not necessarily be applicable to this product.

NCBI GI #
19718751
NCBI GeneID
7374
NCBI Accession #
NP_550433
UniProt Primary Accession #
P13051
UniProt Secondary Accession #
O00637; O00719; Q93028; A8K5M6; B2R8Y1
UniProt Related Accession #
P13051
GenBank Nucleotide #
NM_080911.2
NCBI Official Full Name
uracil-DNA glycosylase isoform UNG2
NCBI Official Synonym Full Names
uracil DNA glycosylase
NCBI Official Symbol
UNG
NCBI Official Synonym Symbols
DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15
UniProt Protein Name
Uracil-DNA glycosylase
Protein Family
Probable uracil-DNA glycosylase
UniProt Gene Name
UNG
UniProt Synonym Gene Names
UDG
NCBI Protein Information
uracil-DNA glycosylase

NCBI Summary for UNG

This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]
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UniProt Comments for UNG

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Research Articles on UNG

1. UDG depleted cancer cells displayed sustained DNA damage following 5-FdU treatment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UNG antibody

 Reference Product  PubMed Publications
 DUT antibody  >25 publications with UNG and DUT
 PCNA antibody  >14 publications with UNG and PCNA
 APEX1 antibody  >12 publications with UNG and APEX1
 OGG1 antibody  >7 publications with UNG and OGG1
 FEN1 antibody  >4 publications with UNG and FEN1
 LIG1 antibody  >3 publications with UNG and LIG1

Pathways associated with anti-UNG antibody

 Products by Pathway  Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base-Excision Repair, AP Site Formation Pathway antibodies  Base-Excision Repair, AP Site Formation Pathway Diagram
 Cleavage Of The Damaged Pyrimidine Pathway antibodies  Cleavage Of The Damaged Pyrimidine Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Depyrimidination Pathway antibodies  Depyrimidination Pathway Diagram
 Displacement Of DNA Glycosylase By APEX1 Pathway antibodies  Displacement Of DNA Glycosylase By APEX1 Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram

Diseases associated with anti-UNG antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >87 publications with UNG and Nervous System Diseases
 Breast Neoplasms Antibodies  >73 publications with UNG and Breast Neoplasms
 Brain Diseases Antibodies  >54 publications with UNG and Brain Diseases
 Lung Neoplasms Antibodies  >52 publications with UNG and Lung Neoplasms
 Disease Models, Animal Antibodies  >32 publications with UNG and Disease Models, Animal
 Inflammation Antibodies  >29 publications with UNG and Inflammation
 Adenocarcinoma Antibodies  >28 publications with UNG and Adenocarcinoma
 Necrosis Antibodies  >25 publications with UNG and Necrosis
 Heart Diseases Antibodies  >19 publications with UNG and Heart Diseases
 Colorectal Neoplasms Antibodies  >19 publications with UNG and Colorectal Neoplasms

Organs/Tissues associated with anti-UNG antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >184 publications with UNG and Blood
 Brain Antibodies  >86 publications with UNG and Brain
 Lung Antibodies  >75 publications with UNG and Lung
 Bone Antibodies  >51 publications with UNG and Bone
 Lymph Node Antibodies  >49 publications with UNG and Lymph Node
 Lymph Antibodies  >44 publications with UNG and Lymph
 Liver Antibodies  >34 publications with UNG and Liver
 Eye Antibodies  >30 publications with UNG and Eye
 Heart Antibodies  >29 publications with UNG and Heart
 Muscle Antibodies  >27 publications with UNG and Muscle
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