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SERPINA12 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200065

Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Vaspin (Serpina12), Monoclonal Antibody
Full Product Name
Vaspin antibody
Product Gene Name
Product Synonym Names
Serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12; SERPINA12; serpin peptidase inhibitor clade A member 12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Serpin A12 precursor; OL64 OL 64; ENSG00000165953; Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 antibody
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
1C4
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
47,527 Da
Antigen Species
Human
Immunogen
Recombinant human Vaspin (21-414aa) purified from E. coli
Clone Note
Anti-human Vaspin mAb, clone 1C4, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Vaspin protein.

Product Categories/Family for anti-Serpina12 antibody

Applications Tested/Suitable for anti-Serpina12 antibody
ELISA (EIA), Western Blot (WB) (Cell lysate)
Application Notes for anti-Serpina12 antibody
WB: 1:1,000 ~ 3,000.
Recommended starting dilution is 1:2,000.

Western Blot (WB) of anti-Serpina12 antibody

Rat serum (15ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Vaspin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-SERPINA12 Monoclonal Antibody – Cat# MBS200065

Product References and Citations for anti-Serpina12 antibody

Hida K, et al., (2005) Natl Acad Sci 102 (30):10610-5; Klotinq N, et al., (2006) Biochem 339 (1):430-6

Other Notes

Small volumes of anti-Serpina12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Serpina12 purchase

MBS200065 is an antibody targeting Serpina12. Also known as Vaspin antibody. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Serpina12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
visceral adipose tissue specific SERPIN
UniProt Protein Name
Serpin A12
UniProt Synonym Protein Names
Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin
Protein Family
UniProt Gene Name
Serpina12
UniProt Synonym Gene Names
Vaspin

UniProt Comments for Serpina12

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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