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NSD3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200067

This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of… More details

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Product Overview

Online Datasheet in PDF
Product Name
NSD3, Monoclonal Antibody
Full Product Name
NSD3 antibody
Product Gene Name
Product Synonym Names
Transcription elongation factor B polypeptide 3 binding protein 1; DKFZp667H044; FLJ20353; Histone lysine N methyltransferase NSD3; MGC126766; MGC142029; NSD 3; Nuclear set domain containing 3; Nuclear SET domain containing protein 3; pp14328; Whistle; WHSC1 like protein 1; WHSC1L1 protein; Wolf Hirschhorn syndrome candidate 1 like 1
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT7B4
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
160,314 Da
OMIM
AF332468 mRNA
3D Structure
ModBase 3D Structure for Q9BZ95
Antigen Species
Human
Immunogen
Recombinant human NSD3 (383-660aa) purified from E. coli
Clone Note
Anti-human NSD3 mAb, clone AT7B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NSD3 protein.

Product Categories/Family for anti-NSD3 antibody

Applications Tested/Suitable for anti-NSD3 antibody
ELISA (EIA), Western Blot (WB)

Western Blot (WB) of anti-NSD3 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: NIH3T3 cell lysate Lane 5.: U87MG cell lysate
Mouse Anti-NSD3 Monoclonal Antibody – Cat# MBS200067

Product References and Citations for anti-NSD3 antibody

Angrand P.O, et al. (2001) Genomics 74 (1):79-88; Stec I, et al. (2000) FEBS Lett 473 (1):1-5

Other Notes

Small volumes of anti-NSD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NSD3 purchase

MBS200067 is an antibody targeting NSD3. Also known as NSD3 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NSD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone-lysine N-methyltransferase NSD3 isoform long
NCBI Official Synonym Full Names
nuclear receptor binding SET domain protein 3
NCBI Official Symbol
NSD3
NCBI Official Synonym Symbols
KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
UniProt Protein Name
Histone-lysine N-methyltransferase NSD3
UniProt Synonym Protein Names
Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; Wolf-Hirschhorn syndrome candidate 1-like protein 1; WHSC1-like protein 1
UniProt Gene Name
NSD3
UniProt Synonym Gene Names
WHSC1-like protein 1
NCBI Protein Information
histone-lysine N-methyltransferase NSD3

NCBI Summary for NSD3

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]
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UniProt Comments for NSD3

Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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