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CD73 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200071

CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their… More details

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Product Overview

Online Datasheet in PDF
Product Name
CD73, Monoclonal Antibody
Full Product Name
CD73 antibody
Product Gene Name
Product Synonym Names
5' nucleotidase; ecto; 5'-nucleotidase ecto (CD73); 5' nucleotidase (CD73); CD73; eN; eNT
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT89E9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
57,949 Da
OMIM
X55740 mRNA
3D Structure
ModBase 3D Structure for P21589
Antigen Species
Human
Immunogen
Recombinant human CD73 (27-252aa) purified from E. coli
Clone Note
Anti-human CD73 mAb, clone AT89E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD73 protein.

Product Categories/Family for anti-CD73 antibody

Applications Tested/Suitable for anti-CD73 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-CD73 antibody
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-CD73 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: WiDr cell lysate Lane 3.: A431 cell lysate
Mouse Anti-CD73 Monoclonal Antibody – Cat# MBS200071

Immunofluorescence (IF) of anti-CD73 antibody

ICC/IF analysis of CD73 in HeLa cells. The cell was stained with ACD0806 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-CD73 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200071

Product References and Citations for anti-CD73 antibody

Sern P.C, et al., (2006) Purinergic Signal 2 (2):351-360; Linda F.T, et al., (2004) J Exp Med 200 (11):1395-1405; Laura A, et al., (1997) J Cell Biol 136 (2):421-431

Other Notes

Small volumes of anti-CD73 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD73 purchase

MBS200071 is an antibody targeting CD73. Also known as CD73 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD73. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
5'-nucleotidase isoform 1 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase ecto
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
NCBI Protein Information
5'-nucleotidase

NCBI Summary for CD73

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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UniProt Comments for CD73

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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