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Visfatin (NAMPT) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200079

Visfatin is pre-B cell colony-enhancing factor 1 (PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil… More details

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Product Overview

Online Datasheet in PDF
Product Name
Visfatin (NAMPT), Monoclonal Antibody
Full Product Name
Visfatin antibody
Product Gene Name
Product Synonym Names
Nicotinamide phosphoribosyltransferase precursor; Pre-B-cell colony-enhancing factor 1; NAMPT; MGC117256; Nicotinamide phosphoribosyltransferase precursor; PBEF; PBEF1; VF; NAMPT; Visfatin; Nicotinamide phosphoribosyltransferase precursor; pre-B-cell colony enhancing factor 1
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
4D5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
55,521 Da
OMIM
U02020 mRNA
3D Structure
ModBase 3D Structure for P43490
Antigen Species
Human
Immunogen
Recombinant human Visfatin (1-491aa) purified from E. coli
Clone Note
Anti-human Visfatin mAb, clone 4D5, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Visfatin protein.

Product Categories/Family for anti-NAMPT antibody

Applications Tested/Suitable for anti-NAMPT antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NAMPT antibody
WB: 1:1,000~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-NAMPT antibody

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate (30ug) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : SW480 cell lysate (40ug)
Mouse Anti-NAMPT Monoclonal Antibody – Cat# MBS200079

Immunofluorescence (IF) of anti-NAMPT antibody

ICC/IF analysis of Visfatin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Visfatin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-NAMPT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200079

Product References and Citations for anti-NAMPT antibody

Stephens JM, et al., (2006) Curr Opin Lipidol. 17:128-31; Hug C, et al., (2005) Science. 307:366-367

Other Notes

Small volumes of anti-NAMPT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAMPT purchase

MBS200079 is an antibody targeting NAMPT. Also known as Visfatin antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NAMPT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Synonym Protein Names
Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
NCBI Protein Information
nicotinamide phosphoribosyltransferase

NCBI Summary for NAMPT

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]
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UniProt Comments for NAMPT

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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