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Cyclophilin B (PPIB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200088

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to… More details

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Product Overview

Online Datasheet in PDF
Product Name
Cyclophilin B (PPIB), Monoclonal Antibody
Full Product Name
Cyclophilin B antibody
Product Gene Name
anti-PPIB antibody
Product Synonym Names
PPIB; Peptidylprolyl isomerase B; CYPB; SCYLP; CYP-S1: Cyclophilin-B; Cyclophilin B; 26- 216aa; AA553318; AI844835; Cphn 2; Cphn2; CyP 20b; AA408962; CYP S1; MGC14109; MGC2224; peptidyl prolyl cis trans isomerase B; Peptidyl prolyl cis trans isomerase B precursor; PPIase; Rotamase; S cyclophilin.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
k2E2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23,743 Da
OMIM
M63573 mRNA
3D Structure
ModBase 3D Structure for P23284
Antigen Species
Human
Immunogen
Recombinant human Cyclophilin B (26-216aa) purified from E. coli
Clone Note
Anti-human Cyclophilin B mAb, clone k2E2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cyclophilin B protein.

Product Categories/Family for anti-PPIB antibody

Signal Transduction
Applications Tested/Suitable for anti-PPIB antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PPIB antibody
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-PPIB antibody

Cell lysates of HepG2 (30ug) and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: K562 cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: SK-OV-3 cell lysate Lane 7.: LnCap cell lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Cyclophilin B Recombinant Protein Lane 2.: Cyclophilin A Recombinant Protein Lane 3.: Cyclophilin D Recombinant Protein Lane 4.: Cyclophilin E Recombinant Protein Lane 5.: Cyclophilin F Recombinant Protein Lane 6.: Cyclophilin G Recombinant Protein Lane 7.: Cyclophilin H Recombinant Protein
Mouse Anti-PPIB Monoclonal Antibody – Cat# MBS200088

Immunofluorescence (IF) of anti-PPIB antibody

ICC/IF analysis of Cyclophilin B in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Cyclophilin B antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PPIB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200088

Product References and Citations for anti-PPIB antibody

Watashi K, et al., (2005) Mol Cell 19 (1):111-112; Yurchenko V, et al., (2001) Biochem Biophys Res Commun 288 (4):786-788; E.Roydon P, et al., (1991) Proc Natl Acad Sci USA 88:1903-1907

Other Notes

Small volumes of anti-PPIB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPIB purchase

MBS200088 is an antibody targeting PPIB. Also known as Cyclophilin B antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIB. It may not necessarily be applicable to this product.

NCBI GI #
4758950
NCBI GeneID
5479
NCBI Accession #
NP_000933
UniProt Primary Accession #
P23284
UniProt Secondary Accession #
Q6IBH5; Q9BVK5; A8K534
UniProt Related Accession #
P23284
GenBank Nucleotide #
NM_000942.4
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase B
NCBI Official Synonym Full Names
peptidylprolyl isomerase B
NCBI Official Symbol
PPIB
NCBI Official Synonym Symbols
OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase B
UniProt Synonym Protein Names
CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP
Protein Family
Probable peptidyl-prolyl cis-trans isomerase
UniProt Gene Name
PPIB
UniProt Synonym Gene Names
CYPB; PPIase B; SCYLP
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase B

NCBI Summary for PPIB

The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]
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UniProt Comments for PPIB

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Research Articles on PPIB

1. PPIB mutations and their associated phenotypes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPIB antibody

 Reference Product  PubMed Publications
 CRTAP antibody  >25 publications with PPIB and CRTAP
 LEPRE1 antibody  >22 publications with PPIB and LEPRE1
 COL1A1 antibody  >14 publications with PPIB and COL1A1
 COL1A2 antibody  >12 publications with PPIB and COL1A2
 SERPINH1 antibody  >9 publications with PPIB and SERPINH1

Pathways associated with anti-PPIB antibody

 Products by Pathway  Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram
 Syndecan-1-mediated Signaling Events Pathway antibodies  Syndecan-1-mediated Signaling Events Pathway Diagram

Diseases associated with anti-PPIB antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >7 publications with PPIB and Nervous System Diseases
 Inflammation Antibodies  >4 publications with PPIB and Inflammation
 Breast Neoplasms Antibodies  >2 publications with PPIB and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with PPIB and Carcinoma, Hepatocellular
 Osteogenesis Imperfecta, Type IX Antibodies  >2 publications with PPIB and Osteogenesis Imperfecta, Type IX
 Cardiovascular Diseases Antibodies  >1 publications with PPIB and Cardiovascular Diseases
 Liver Neoplasms Antibodies  >1 publications with PPIB and Liver Neoplasms
 Disease Models, Animal Antibodies  >1 publications with PPIB and Disease Models, Animal
 Kidney Diseases Antibodies  >1 publications with PPIB and Kidney Diseases
 Heart Diseases Antibodies  >1 publications with PPIB and Heart Diseases

Organs/Tissues associated with anti-PPIB antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >25 publications with PPIB and Bone
 Blood Antibodies  >16 publications with PPIB and Blood
 Skin Antibodies  >12 publications with PPIB and Skin
 Connective Tissue Antibodies  >7 publications with PPIB and Connective Tissue
 Lung Antibodies  >5 publications with PPIB and Lung
 Liver Antibodies  >4 publications with PPIB and Liver
 Brain Antibodies  >4 publications with PPIB and Brain
 Muscle Antibodies  >4 publications with PPIB and Muscle
 Pancreas Antibodies  >3 publications with PPIB and Pancreas
 Heart Antibodies  >3 publications with PPIB and Heart
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