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PPP1R14A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200095

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However,… More details

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Product Overview

Online Datasheet in PDF
Product Name
PPP1R14A, Monoclonal Antibody
Full Product Name
PPP1R14A antibody
Product Gene Name
Product Synonym Names
Protein phosphatase 1 regulatory (inhibitor) subunit 14A; CPI17; CPI-17; PPP1INL; Protein phosphatase 1; regulatory (inhibitor) subunit 14A; PPP1R14A; Protein phosphatase 1; regulatory (inhibitor) subunit 14A CPI 17; CPI 17 alpha; CPI 17alpha; CPI17; PKC potentiated inhibitory protein of PP1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
4H10
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
13,480 Da
OMIM
AB056508 mRNA
3D Structure
ModBase 3D Structure for Q96A00
Antigen Species
Human
Immunogen
Recombinant human PPP1R14A (1-147aa) purified from E. coli
Clone Note
Anti-human PPP1R14A mAb, clone 4H10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP1R14A protein.

Product Categories/Family for anti-PPP1R14A antibody

Applications Tested/Suitable for anti-PPP1R14A antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PPP1R14A antibody
WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.

Western Blot (WB) of anti-PPP1R14A antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : NIH-3T3 cell lysate
Mouse Anti-PPP1R14A Monoclonal Antibody – Cat# MBS200095

Immunofluorescence (IF) of anti-PPP1R14A antibody

ICC/IF analysis of PPP1R14A in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1R14A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PPP1R14A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200095

Product References and Citations for anti-PPP1R14A antibody

Hamaguchi., et al. (2000) Biochem. Biophys. Res Commun. 274 (3):825-30; Eto M., et al. (1997) FEBS Lett. 410 (2-3):356-60; Lartey I., et al. (2007) Biol Reprod. 76 (6):971-82

Other Notes

Small volumes of anti-PPP1R14A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPP1R14A purchase

MBS200095 is an antibody targeting PPP1R14A. Also known as PPP1R14A antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP1R14A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 14A isoform 1
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory inhibitor subunit 14A
NCBI Official Symbol
PPP1R14A
NCBI Official Synonym Symbols
CPI17; CPI-17; PPP1INL
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 14A
UniProt Synonym Protein Names
17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17
UniProt Gene Name
PPP1R14A
UniProt Synonym Gene Names
CPI17; PPP1INL; CPI-17
NCBI Protein Information
protein phosphatase 1 regulatory subunit 14A

NCBI Summary for PPP1R14A

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]
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UniProt Comments for PPP1R14A

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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