• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TMEFF2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200115

TMEFF2 (transmembrane protein with EGF-like and two follistatin-like domains 2), a gene encoding a plasma membrane protein with two follistatin-like domains and one epidermal growth factor-like domain, had limited normal tissue distribution and was highly… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
TMEFF2, Monoclonal Antibody
Full Product Name
TMEFF2 antibody
Product Gene Name
Product Synonym Names
transmembrane protein with EGF-like and twofollistatin-like domains 2; TENB2; HPP1; TR; TPEF; CT120.2; transmembrane protein TENB2; tomoregulin; cancer/testis antigen family 120 member 2
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
J4B6
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
19,472 Da
OMIM
AB017269 mRNA
3D Structure
ModBase 3D Structure for Q9UIK5
Antigen Species
Human
Immunogen
Recombinant human TMEFF2 (41-150aa) purified from E. coli
Clone Note
Anti-human TMEFF2 mAb, clone J4B6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TMEFF2 protein.

Product Categories/Family for anti-TMEFF2 antibody

Applications Tested/Suitable for anti-TMEFF2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-TMEFF2 antibody
Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

Western Blot (WB) of anti-TMEFF2 antibody

The Cell and Tissue lysates (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TMEFF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse brain tissue lysate Lane 2.: HeLa cell lysate The Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TMEFF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: SW480 cell lysate
Mouse Anti-TMEFF2 Monoclonal Antibody – Cat# MBS200115

Immunohistochemistry (IHC) of anti-TMEFF2 antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human TMEFF2 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Mouse Anti-TMEFF2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200115

Product References and Citations for anti-TMEFF2 antibody

Daniel E.H, et al., (2004) Mol Cancer Ther, 3 (8) :921-32.; Quayle S N, et al., (2006) Genomics.; 87 (5):633-7.

Other Notes

Small volumes of anti-TMEFF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TMEFF2 purchase

MBS200115 is an antibody targeting TMEFF2. Also known as TMEFF2 antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TMEFF2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tomoregulin-2 isoform 1
NCBI Official Synonym Full Names
transmembrane protein with EGF like and two follistatin like domains 2
NCBI Official Symbol
TMEFF2
NCBI Official Synonym Symbols
TR; HPP1; TPEF; TR-2; TENB2; CT120.2
UniProt Protein Name
Tomoregulin-2
UniProt Synonym Protein Names
Hyperplastic polyposis protein 1; Transmembrane protein with EGF-like and two follistatin-like domains
Protein Family
UniProt Gene Name
TMEFF2
UniProt Synonym Gene Names
HPP1; TENB2; TPEF; TR-2
NCBI Protein Information
tomoregulin-2

NCBI Summary for TMEFF2

This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Show more

UniProt Comments for TMEFF2

May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TMEFF2 antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000