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TREM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206898

The triggering receptor expressed on myeloid cells-2 (TREM2) is a membrane protein that interacts with the TYROBP protein. The TREM2 and TYROBP proteins form a complex that transmits chemical signals to activate the cell. This complex is involved in the… More details

$295 / 0.05 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
TREM2, Monoclonal Antibody
Full Product Name
Human TREM2 antibody
Product Gene Name
Product Synonym Names
Ttriggering receptor expressed on myeloid cells 2 isoform 1; Triggering receptor expressed on myeloid cells 2; Triggering receptor expressed on myeloid cells 2a; TREM-2; Trem2a; Trem2b; Trem2c
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT1E7
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
3D Structure
ModBase 3D Structure for Q9NZC2
Immunogen
Recombinant human TREM2 (19-161aa) purified from E. coli
Applications Tested/Suitable for anti-TREM2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), FACS
Application Notes for anti-TREM2 antibody
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-TREM2 antibody

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : 293T cell lysate
Lane 2 : TREM2 19-161aa Transfected 293T cell lysate
Mouse Anti-TREM2 Monoclonal Antibody – Cat# MBS206898

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-TREM2 antibody

ICC/IF analysis of TREM2 in HepG2 cells. The cell was stained with MBS206898 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-TREM2 Monoclonal Antibody, Immunocytochemistry (ICC)/Immunofluorescence – Cat# MBS206898

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-TREM2 antibody

ICC/IF analysis of TREM2 in Raw264.7 cells. The cell was stained with MBS206898 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-TREM2 Monoclonal Antibody, Immunocytochemistry (ICC)/Immunofluorescence – Cat# MBS206898

Flow Cytometry (FC/FACS) of anti-TREM2 antibody

Flow cytometry analysis of TREM2 in Hep3B cells. The cell was stained with MBS206898 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-TREM2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS206898

Product References and Citations for anti-TREM2 antibody

N.Diaye EN, et al., (2009) J Cell Biol. 184(2):215-23.
Piccio L, et al., (2008) Brain. 131(11):3081-91.

Other Notes

Small volumes of anti-TREM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TREM2 purchase

MBS206898 is an antibody targeting TREM2. Also known as Human TREM2 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TREM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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