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BID Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200123

BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into the outer mitochondrial membrane. Bax is believed to induce the… More details

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Product Overview

Online Datasheet in PDF
Product Name
BID, Monoclonal Antibody
Full Product Name
BID antibody
Product Gene Name
Product Synonym Names
BH3 interacting domain death agonist isoform 2; FP497; MGC15319; MGC42355; BH3 interacting domain death agonist isoform 2; BID; BH3 interacting domain death agonist isoform 2 Apoptotic death agonist; Apoptotic death agonist BID; BH3 interacting domain death agonist; HGNC:1050; BH3 interacting domain death agonist p11; BH3 interacting domain death agonist p13; BH3 interacting domain death agonist p15; BID isoform ES (1b); BID isoform L (2); Desmocollin type 4; Human BID coding sequence; OTTHuMP00000196197; p11 BID; p13 BID; p15 BID; p22 BID.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
4D3
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
11,263 Da
OMIM
AF042083 mRNA
3D Structure
ModBase 3D Structure for P55957
Antigen Species
Human
Immunogen
Recombinant human BID (1-195aa) purified from E. coli
Clone Note
Anti-human BID mAb, clone 4D3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BID protein.

Product Categories/Family for anti-BID antibody

Applications Tested/Suitable for anti-BID antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-BID antibody
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-BID antibody

The lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BID (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system
Mouse Anti-BID Monoclonal Antibody – Cat# MBS200123

Immunofluorescence (IF) of anti-BID antibody

ICC/IF analysis of BID in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BID antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-BID Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200123

Product References and Citations for anti-BID antibody

Zha J., et al. (2000) Science. 290:1761; Luo X., et al. (1998) Cell. 94:481; Li H., et al. (1998) Cell. 94:491

Other Notes

Small volumes of anti-BID antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BID purchase

MBS200123 is an antibody targeting BID. Also known as BID antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BID. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
BH3-interacting domain death agonist isoform 2
NCBI Official Synonym Full Names
BH3 interacting domain death agonist
NCBI Official Symbol
BID
NCBI Official Synonym Symbols
FP497
UniProt Protein Name
BH3-interacting domain death agonist
UniProt Synonym Protein Names
p22 BID; BID
UniProt Gene Name
BID
UniProt Synonym Gene Names
BID
NCBI Protein Information
BH3-interacting domain death agonist

NCBI Summary for BID

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
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UniProt Comments for BID

The major proteolytic product p15 BID allows the release of cytochrome c (). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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