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WNT3A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200124

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal… More details

$285 / 0.05 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Wnt3a, Monoclonal Antibody
Full Product Name
Wnt3a antibody
Product Gene Name
Product Synonym Names
wingless-type MMTV integration site family; member 3A
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
3A6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
42,905 Da
OMIM
AB060284 mRNA
3D Structure
ModBase 3D Structure for P56704
Antigen Species
Human
Immunogen
Recombinant human Wnt3a (19-352aa) purified from E. coli
Clone Note
Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Product Categories/Family for anti-Wnt3a antibody

Applications Tested/Suitable for anti-Wnt3a antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Wnt3a antibody
Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

Western Blot (WB) of anti-Wnt3a antibody

The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-WNT3A Monoclonal Antibody – Cat# MBS200124

Immunohistochemistry (IHC) of anti-Wnt3a antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Mouse Anti-WNT3A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200124

Product References and Citations for anti-Wnt3a antibody

Pan W., et al. (2008) Science. 321 (5894):1350-1353; Katoh M., et al. (2002) Int J Oncol. 20 (2):373-377; Smolich B D., et al. (1993) Mol Biol Cell. 4 (12):1267-1275

Other Notes

Small volumes of anti-Wnt3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Wnt3a purchase

MBS200124 is an antibody targeting Wnt3a. Also known as Wnt3a antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Wnt3a. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein Wnt-3a
NCBI Official Synonym Full Names
Wnt family member 3A
NCBI Official Symbol
WNT3A
UniProt Protein Name
Protein Wnt-3a
UniProt Gene Name
WNT3A
NCBI Protein Information
protein Wnt-3a

NCBI Summary for Wnt3a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
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UniProt Comments for Wnt3a

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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