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FABP7 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200130

FABP7 (Fatty acid binding protein 7) is also known as brain fatty acid-binding protein (B-FABP) and is a member of fatty acid-binding proteins (FABPs) which are a family of small, highly conserved, cytoplasmic proteins to bind long-chain fatty acids and other… More details

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Product Overview

Online Datasheet in PDF
Product Name
FABP7, Monoclonal Antibody
Full Product Name
FABP7 antibody
Product Gene Name
Product Synonym Names
Fatty acid binding protein 7; Fatty acid binding protein 7; FABP7; Fatty acid binding protein 7 B FABP; BFABP; BLBP; Brain lipid binding protein; DKFZp547J2313; FABP 7; FABPB; Fatty acid binding protein 7 brain; Fatty acid binding protein brain; OTTHuMP00000017119; Mammary derived growth inhibitor related; MRG.
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1D1
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
18,829 Da
OMIM
AJ002962 mRNA
3D Structure
ModBase 3D Structure for O15540
Antigen Species
Human
Immunogen
Recombinant human FABP7 (1-132aa) purified from E. coli
Clone Note
Anti-human FABP7 mAb, clone AT1D1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FABP7 protein.

Product Categories/Family for anti-FABP7 antibody

Applications Tested/Suitable for anti-FABP7 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-FABP7 antibody
Usage: The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-FABP7 antibody

The Mouse tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP7 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Testis tissue lysate
Mouse Anti-FABP7 Monoclonal Antibody – Cat# MBS200130

Western Blot (WB) of anti-FABP7 antibody

The tissue lysate of mouse brain (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-FABP7 Monoclonal Antibody, Western Blot (WB) – Cat# MBS200130

Immunflorescense (IF) of anti-FABP7 antibody

ICC/IF analysis of FABP7 in U87MG cells. The cell was stained with MBS200130 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-FABP7 Monoclonal Antibody, Immunflorescense (IF) – Cat# MBS200130

Immunflorescense (IF) of anti-FABP7 antibody

ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with MBS200130 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-FABP7 Monoclonal Antibody, Immunflorescense (IF) – Cat# MBS200130

Product References and Citations for anti-FABP7 antibody

Borchers T, et al., (1997) Prostaglandins Leukot. Essent. Fatty Acids. 57 (1):77-84; Liu Rz, et al., (2003) Eur J Biochem. 270 (4):715-25

Other Notes

Small volumes of anti-FABP7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FABP7 purchase

MBS200130 is an antibody targeting FABP7. Also known as FABP7 antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FABP7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
fatty acid-binding protein, brain isoform 1
NCBI Official Synonym Full Names
fatty acid binding protein 7
NCBI Official Symbol
FABP7
NCBI Official Synonym Symbols
MRG; BLBP; FABPB; B-FABP
UniProt Protein Name
Fatty acid-binding protein, brain
UniProt Synonym Protein Names
Brain lipid-binding protein; BLBP; Brain-type fatty acid-binding protein; B-FABP; Fatty acid-binding protein 7; Mammary-derived growth inhibitor related
UniProt Gene Name
FABP7
UniProt Synonym Gene Names
BLBP; FABPB; MRG; BLBP; B-FABP
NCBI Protein Information
fatty acid-binding protein, brain

NCBI Summary for FABP7

The gene encodes a small, highly conserved cytoplasmic protein that bind long-chain fatty acids and other hydrophobic ligands. The encoded protein is important in the establishment of the radial glial fiber in the developing brain. Alternative splicing and promoter usage results in multiple transcript variants encoding different isoforms. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Jan 2016]
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UniProt Comments for FABP7

B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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