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14-3-3 tau Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200131

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms,beta,… More details

$285 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
14-3-3 tau, Monoclonal Antibody
Full Product Name
14-3-3 tau antibody
Product Gene Name
anti-14-3-3 tau antibody
Product Synonym Names
tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein theta polypeptide; YWHAQ; 1C5; HS1; 14-3-3 tau; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; theta polypeptide; tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; theta polypeptide 14 3 3 protein T cell; 14 3 3 protein tau; 14 3 3 protein theta; 14 3 3 tau; 14 3 3 theta; KCIP1; Protein HS1; Protein kinase C inhibitor protein 1; tyr3/trp5 monooxygenase activation protein; theta; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; theta polypeptide.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT1A1
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
27,764 Da
OMIM
X56468 mRNA
3D Structure
ModBase 3D Structure for P27348
Antigen Species
Human
Immunogen
Recombinant human 14-3-3 tau (1-245aa) purified from E. coli
Clone Note
Anti-human 14-3-3 tau mAb, clone AT1A1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-3 tau protein.

Product Categories/Family for anti-14-3-3 tau antibody

Signal Transduction
Applications Tested/Suitable for anti-14-3-3 tau antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-14-3-3 tau antibody
WB: 1:1000 ~ 2000.
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-14-3-3 tau antibody

Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human YWHAZ Lane 2. : Recombinant Human YWHAB Lane 3. : Recombinant Human YWHAE Lane 4. : Recombinant Human YWHAH Lane 5. : Recombinant Human YWHAG Lane 6. : Recombinant Human SFN Lane 7. : Recombinant Human YWHAQ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MDA-MB-21 cell lysate Lane 2. : HeLa cell lysate Lane 3. : A431 cell lysate Lane 4. : NIH3T3 cell lysate Lane 5. : 293T cell lysate Lane 6. : HepG2 cell lysate
Mouse Anti-14-3-3 tau Monoclonal Antibody – Cat# MBS200131

Immunofluorescence (IF) of anti-14-3-3 tau antibody

ICC/IF analysis of 14-3-3 tau in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 tau antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-14-3-3 tau Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200131

Product References and Citations for anti-14-3-3 tau antibody

Liu YC, et al., (1996) J Biol Chem 271:14591-14595; Xiao B, et al., (1995) Nature 376:188-191

Other Notes

Small volumes of anti-14-3-3 tau antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 14-3-3 tau purchase

MBS200131 is an antibody targeting 14-3-3 tau. Also known as 14-3-3 tau antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for 14-3-3 tau. It may not necessarily be applicable to this product.

NCBI GI #
5803227
NCBI GeneID
10971
NCBI Accession #
NP_006817
UniProt Primary Accession #
P27348
UniProt Secondary Accession #
Q567U5; Q5TZU8; Q9UP48; D6W4Z5
UniProt Related Accession #
P27348
GenBank Nucleotide #
NM_006826.3
NCBI Official Full Name
14-3-3 protein theta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
NCBI Official Symbol
YWHAQ
NCBI Official Synonym Symbols
1C5; HS1; 14-3-3
UniProt Protein Name
14-3-3 protein theta
UniProt Synonym Protein Names
14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1
Protein Family
Glutathione S-transferase
UniProt Gene Name
YWHAQ
NCBI Protein Information
14-3-3 protein theta

NCBI Summary for 14-3-3 tau

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]
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UniProt Comments for 14-3-3 tau

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
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Research Articles on 14-3-3 tau

1. Loss of Par3 promotes metastatic behavior in lung adenocarcinoma cells through 14-3-3zeta protein.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-14-3-3 tau antibody

 Reference Product  PubMed Publications
 YWHAZ antibody  >3 publications with 14-3-3 tau and YWHAZ
 YWHAH antibody  >2 publications with 14-3-3 tau and YWHAH
 YWHAB antibody  >2 publications with 14-3-3 tau and YWHAB

Pathways associated with anti-14-3-3 tau antibody

 Products by Pathway  Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway antibodies  Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway Diagram

Diseases associated with anti-14-3-3 tau antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with 14-3-3 tau and Carcinoma
 Neoplasm Metastasis Antibodies  >1 publications with 14-3-3 tau and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >1 publications with 14-3-3 tau and Adenocarcinoma
 Disease Models, Animal Antibodies  >1 publications with 14-3-3 tau and Disease Models, Animal
 Breast Neoplasms Antibodies  >1 publications with 14-3-3 tau and Breast Neoplasms

Organs/Tissues associated with anti-14-3-3 tau antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with 14-3-3 tau and Brain
 Heart Antibodies  >4 publications with 14-3-3 tau and Heart
 Nerve Antibodies  >4 publications with 14-3-3 tau and Nerve
 Muscle Antibodies  >4 publications with 14-3-3 tau and Muscle
 Blood Antibodies  >3 publications with 14-3-3 tau and Blood
 Prostate Antibodies  >2 publications with 14-3-3 tau and Prostate
 Embryonic Tissue Antibodies  >2 publications with 14-3-3 tau and Embryonic Tissue
 Ovary Antibodies  >1 publications with 14-3-3 tau and Ovary
 Liver Antibodies  >1 publications with 14-3-3 tau and Liver
 Kidney Antibodies  >1 publications with 14-3-3 tau and Kidney
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