Mouse Anti-CKB Monoclonal Antibody – Cat# MBS2001328

Western Blot: Sample: Recombinant protein.

Creatine Kinase, Brain (CKB) Antibody – Mouse Monoclonal

Name: CKB Antibody; Monoclonal; Mouse; Rat/Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS2001328
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2001328

MBS2001328 is an antibody targeting CKB. Also known as Monoclonal Antibody to Creatine Kinase, Brain (CKB). Reactive in Rat, Human, Mouse. Host: Mouse. More details

$230 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Creatine Kinase, Brain (CKB), Monoclonal Antibody

Full Product Name

Monoclonal Antibody to Creatine Kinase, Brain (CKB)

Product Gene Name

anti-CKB antibody

Immunogen Sequence

Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-KLRFPA EDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG FTLDDAIQTG VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEDRHGGY QPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLS GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV

Clonality

Monoclonal

Clone Number

2-1#

Host

Mouse

Species Reactivity

Rat, Human, Mouse

Form/Format

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

Purity/Purification

Affinity Chromatography

Concentration

1mg/mL (lot specific)

Specificity

The antibody is a mouse monoclonal antibody raised against CKB. It has been selected for its ability to recognize CKB in immunohistochemical staining andwestern blotting.

Preparation and Storage

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Matching Pairs

Unconjugated Antibody: CKB Clone #2-1# (MBS2001328)
APC-CY7 Conjugated Antibody: CKB Clone #2-1# (MBS2062259)

Matching Pairs

Unconjugated Antibody: CKB Clone #2-1# (MBS2001328)
PE Conjugated Antibody: CKB Clone #2-1# (MBS2062261)

Molecular Weight

42,725 Da

3D Structure

ModBase 3D Structure for P07335

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Immunogen

Recombinant CKB (Lys11~Leu367) expressed in E.coli.

Cross Reactivity

Rat

Conjugated Antibody

The APC conjugated antibody version of this item is also available as catalog #MBS2062263

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for anti-CKB antibody

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Application Notes for anti-CKB antibody

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Other Notes

Small volumes of anti-CKB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CKB. It may not necessarily be applicable to this product.

NCBI GI #

401461784

NCBI GeneID

24264

NCBI Accession #

NP_036661.3

UniProt Primary Accession #

P07335

UniProt Secondary Accession #

Q499P7; Q5PPJ5; Q6IRE0; Q6P139; A0JPK7

UniProt Related Accession #

P07335

GenBank Nucleotide #

NM_012529.3

NCBI Official Full Name

creatine kinase B-type

NCBI Official Synonym Full Names

creatine kinase B

NCBI Official Symbol

Ckb

NCBI Official Synonym Symbols

Ckbb; Ckbr

UniProt Protein Name

Creatine kinase B-type

UniProt Synonym Protein Names

B-CK; Creatine kinase B chain

Protein Family

Creatine kinase

UniProt Gene Name

Ckb

UniProt Synonym Gene Names

Ckbb

NCBI Protein Information

creatine kinase B-type

NCBI Summary for CKB

brain specific isozyme that catalyzed the interconversion of ATP and creatine to ADP and phosphocreatine; may play a role in ATP generation during cerebellar development [RGD, Feb 2006]

UniProt Comments for CKB

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 6q32

Cellular Component: cell soma; cytosol; dendrite; mitochondrion; myelin sheath; nucleus

Molecular Function: ATP binding; creatine kinase activity; protein binding; ubiquitin protein ligase binding

Biological Process: brain development; cellular chloride ion homeostasis; cerebellum development; phosphorylation; substantia nigra development

Research Articles on CKB

1. Data indicate that the beta-alanine administration was able to inhibit the enzyme pyruvate kinase, cytosolic creatine kinase, and adenylate kinase activities in cerebral cortex, and increase in cerebellum.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CKB antibody

Products by Pathway	Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Creatine Metabolism Pathway antibodies	Creatine Metabolism Pathway Diagram
Creatine Pathway antibodies	Creatine Pathway Diagram
Creatine Pathway antibodies	Creatine Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Urea Cycle And Metabolism Of Amino Groups Pathway antibodies	Urea Cycle And Metabolism Of Amino Groups Pathway Diagram

Diseases associated with anti-CKB antibody

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>15 publications with CKB and Brain Diseases
Nervous System Diseases Antibodies	>10 publications with CKB and Nervous System Diseases
Cardiovascular Diseases Antibodies	>8 publications with CKB and Cardiovascular Diseases
Heart Diseases Antibodies	>6 publications with CKB and Heart Diseases
Adenocarcinoma Antibodies	>5 publications with CKB and Adenocarcinoma
Liver Diseases Antibodies	>4 publications with CKB and Liver Diseases
Cognition Disorders Antibodies	>4 publications with CKB and Cognition Disorders
Lung Diseases Antibodies	>3 publications with CKB and Lung Diseases
Hyperplasia Antibodies	>2 publications with CKB and Hyperplasia
Necrosis Antibodies	>2 publications with CKB and Necrosis

Organs/Tissues associated with anti-CKB antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>59 publications with CKB and Brain
Uterus Antibodies	>12 publications with CKB and Uterus
Heart Antibodies	>8 publications with CKB and Heart
Nerve Antibodies	>5 publications with CKB and Nerve
Lung Antibodies	>4 publications with CKB and Lung
Connective Tissue Antibodies	>3 publications with CKB and Connective Tissue
Inner Ear Antibodies	>3 publications with CKB and Inner Ear
Spleen Antibodies	>3 publications with CKB and Spleen
Embryonic Tissue Antibodies	>3 publications with CKB and Embryonic Tissue
Kidney Antibodies	>2 publications with CKB and Kidney