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ORF73/HHV8 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200144

ORF73 protein of human herpesvirus 8 (HHV-8) is a kaposi's sarcoma-associated herpesvirus (kSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ORF73/HHV8, Monoclonal Antibody
Full Product Name
ORF73/HHV8 antibody
Product Gene Name
Product Synonym Names
Human herpesvirus 8 ORF73; Kaposi's sarcoma-associated herpesvirus
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT4C11
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
3D Structure
ModBase 3D Structure for O40947
Antigen Species
Human
Immunogen
Recombinant human ORF73/HHV8 (122-329aa) purified from E. coli
Clone Note
Anti-human ORF73/HHV8 mAb, clone AT4C11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ORF73/HHV8 protein.
Usage
The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Product Categories/Family for anti-ORF73/HHV8 antibody

Applications Tested/Suitable for anti-ORF73/HHV8 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-ORF73/HHV8 antibody

Transfected and non-transfected 293T cell lysates were resolved by SDS-PAGE, transferred to NC membrane and probed with anti- ORF73/HHV8 (1:1,000) and anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-ORF73/HHV8 Monoclonal Antibody – Cat# MBS200144

Immunohistochemistry (IHC) of anti-ORF73/HHV8 antibody

Paraffin embedded sections of Kaposi's sarcoma tissue were incubated with anti-ORF73/HHV8 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Mouse Anti-ORF73/HHV8 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200144

Western Blot (WB) of anti-ORF73/HHV8 antibody

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ORF73/HHV8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate (40ug)
Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug)
Mouse Anti-ORF73/HHV8 Monoclonal Antibody, Western Blot (WB) – Cat# MBS200144

Western Blot (WB) of anti-ORF73/HHV8 antibody

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate (40ug)
Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug)
Mouse Anti-ORF73/HHV8 Monoclonal Antibody, Western Blot (WB) – Cat# MBS200144

Product References and Citations for anti-ORF73/HHV8 antibody

kedes D.H., et al. (1997) Journal of Clinical Investigation. 100: 2610-2606.; Verma SC., et al (2006) Journal of Virology 80: 3445-3458.

Other Notes

Small volumes of anti-ORF73/HHV8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ORF73/HHV8 purchase

MBS200144 is an antibody targeting ORF73/HHV8. Also known as ORF73/HHV8 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ORF73/HHV8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
ORF 73
UniProt Protein Name
ORF 73
UniProt Synonym Protein Names
ORF 73

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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