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HOMER1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200145

Homer1 (Homer protein homolog 1) belongs to Homer family of proteins that regulate signal transduction through, and the trafficking of, glutamate receptors, as well as maintain and regulate extracellular glutamate levels in corticolimbic brain regions. The… More details

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Product Overview

Online Datasheet in PDF
Product Name
Homer1, Monoclonal Antibody
Full Product Name
Homer1 antibody
Product Gene Name
Product Synonym Names
Homer protein homolog 1; HOMER; HOMER1A; HOMER1B; HOMER1C; SYN47; Homer protein homolog 1; Homer 1; Homer protein homolog 1 HOMER 1A; HOMER 1B; HOMER 1C; Homer; neuronal immediate early gene; 1; HOMER1; Ves 1
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1F3
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
19,861 Da
OMIM
AF093262 mRNA
3D Structure
ModBase 3D Structure for Q86YM7
Antigen Species
Human
Immunogen
Recombinant human Homer1 (1-354aa) purified from E. coli
Clone Note
Anti-human Homer1 mAb, clone AT1F3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Homer1 protein.

Product Categories/Family for anti-Homer1 antibody

Applications Tested/Suitable for anti-Homer1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Homer1 antibody
WB: 1:1000 ~ 2000.
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-Homer1 antibody

The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Homer1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-HOMER1 Monoclonal Antibody – Cat# MBS200145

Immunofluorescence (IF) of anti-Homer1 antibody

ICC/IF analysis of Homer1 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Homer1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-HOMER1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200145

Product References and Citations for anti-Homer1 antibody

Szumlinski KK, et al., (2008) Biochem Pharmacol. 75 (1):112-33; Roche KW, et al., (1999) J Biol Chem.274 (36):25953-7

Other Notes

Small volumes of anti-Homer1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Homer1 purchase

MBS200145 is an antibody targeting Homer1. Also known as Homer1 antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Homer1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
homer protein homolog 1 isoform 1
NCBI Official Synonym Full Names
homer scaffolding protein 1
NCBI Official Symbol
HOMER1
NCBI Official Synonym Symbols
HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
UniProt Protein Name
Homer protein homolog 1
Protein Family
UniProt Gene Name
HOMER1
UniProt Synonym Gene Names
SYN47; Homer-1
NCBI Protein Information
homer protein homolog 1

NCBI Summary for Homer1

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq, Jul 2008]

UniProt Comments for Homer1

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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