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Never in Mitosis Gene A Related Kinase 2 (NEK2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001516
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MBS2001516 is an antibody targeting NEK2. Also known as Polyclonal Antibody to Never In Mitosis Gene A Related Kinase 2 (NEK2). Reactive in Rat. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Never In Mitosis Gene A Related Kinase 2 (NEK2), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Never In Mitosis Gene A Related Kinase 2 (NEK2)
Product Gene Name
anti-NEK2 antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-VLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTP YYMSPEQISR LSYNEKSDIW SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLQDSS PVLSELKLKE RQLQDRERAL RAREDSLEQK ERELCIRERL AEDKLARAES LLKNYSLLKE HRLLCLAGGS ELDLPSSALK KKVHFHGESK ENTARSENSE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against NEK2. It has been selected for its ability to recognize NEK2 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: NEK2 (MBS2001516)
Immunogen: NEK2 (MBS2009270)
Matching Pairs
Unconjugated Antibody: NEK2 (MBS2001516)
APC-CY7 Conjugated Antibody: NEK2 (MBS2079967)
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Molecular Weight
45,449 Da
3D Structure
ModBase 3D Structure for Q91XQ1
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant NEK2 (Val137~Glu400) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079969
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NEK2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-NEK2 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-NEK2 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-NEK2 Polyclonal Antibody – Cat# MBS2001516

Immunohistochemistry (IHC) of anti-NEK2 antibody

DABstainingonIHC-P.Samples:RatTissue)
Rabbit Anti-NEK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001516

Other Notes

Small volumes of anti-NEK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK2. It may not necessarily be applicable to this product.

NCBI GI #
281371462
NCBI GeneID
114482
NCBI Accession #
NP_446143.1
UniProt Primary Accession #
Q91XQ1
GenBank Nucleotide #
NM_053691.2
NCBI Official Full Name
serine/threonine-protein kinase Nek2
NCBI Official Synonym Full Names
NIMA-related kinase 2
NCBI Official Symbol
Nek2
UniProt Protein Name
NIMA related kinase 2
UniProt Synonym Protein Names
NIMA related kinase 2
Protein Family
Probable serine/threonine-protein kinase
UniProt Gene Name
Nek2
NCBI Protein Information
serine/threonine-protein kinase Nek2

NCBI Summary for NEK2

mouse homolog displays cell cycle associated changes in nuclear and cytoplasmic localization; may act to regulate mitosis [RGD, Feb 2006]

UniProt Comments for NEK2

NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation

Protein type: EC 2.7.11.1; Kinase, protein; NEK family; Nucleolus; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 13q27

Cellular Component: centrosome; condensed nuclear chromosome; kinetochore; midbody; nucleoplasm; protein complex; spindle pole

Molecular Function: protein kinase activity; protein phosphatase binding

Biological Process: blastocyst development; centrosome separation; chromosome segregation; mitotic sister chromatid segregation; mitotic spindle assembly; negative regulation of DNA binding; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; protein amino acid phosphorylation; protein autophosphorylation; regulation of attachment of spindle microtubules to kinetochore; regulation of mitotic centrosome separation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEK2 antibody

 Reference Product  PubMed Publications
 Cdc2 antibody  >14 publications with NEK2 and Cdc2
 Cdc20 antibody  >6 publications with NEK2 and Cdc20
 Ndc80 antibody  >5 publications with NEK2 and Ndc80
 Top2a antibody  >3 publications with NEK2 and Top2a
 Hmga2 antibody  >3 publications with NEK2 and Hmga2
 Bub1b antibody  >3 publications with NEK2 and Bub1b
 Cenpf antibody  >1 publications with NEK2 and Cenpf

Pathways associated with anti-NEK2 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 G2/M Transition Pathway antibodies  G2/M Transition Pathway Diagram
 Loss Of Nlp From Mitotic Centrosomes Pathway antibodies  Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
 Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway antibodies  Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway Diagram
 Mitotic G2-G2/M Phases Pathway antibodies  Mitotic G2-G2/M Phases Pathway Diagram
 Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway antibodies  Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway Diagram
 Regulation Of PLK1 Activity At G2/M Transition Pathway antibodies  Regulation Of PLK1 Activity At G2/M Transition Pathway Diagram

Diseases associated with anti-NEK2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >45 publications with NEK2 and Neoplasms
 Breast Neoplasms Antibodies  >19 publications with NEK2 and Breast Neoplasms
 Carcinoma Antibodies  >14 publications with NEK2 and Carcinoma
 Adenocarcinoma Antibodies  >11 publications with NEK2 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >10 publications with NEK2 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >4 publications with NEK2 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >4 publications with NEK2 and Nervous System Diseases
 Lung Neoplasms Antibodies  >3 publications with NEK2 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3 publications with NEK2 and Carcinoma, Hepatocellular
 Kidney Neoplasms Antibodies  >3 publications with NEK2 and Kidney Neoplasms

Organs/Tissues associated with anti-NEK2 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >8 publications with NEK2 and Testis
 Lung Antibodies  >7 publications with NEK2 and Lung
 Heart Antibodies  >2 publications with NEK2 and Heart
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