• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Glypican 4 (GPC4) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001599
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2001599 is an antibody targeting GPC4. Also known as Polyclonal Antibody to Glypican 4 (GPC4). Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$205 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glypican 4 (GPC4), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Glypican 4 (GPC4)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-T FAQGLAVARD VVSKVSVVNP TAQCTHALLK MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDFEWNN FIDAMLMVAE RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG RISRSISESA FSARFRPYHP EQRPTTAAGT SLDRLVTDVK EKLKQAKKFW SSLPSTVCND ERMAAGNENE DDCWNG
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against GPC4. It has been selected for its ability to recognize GPC4 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: GPC4 (MBS2001599)
Immunogen: GPC4 (MBS2011505)
Matching Pairs
Unconjugated Antibody: GPC4 (MBS2001599)
APC-CY7 Conjugated Antibody: GPC4 (MBS2050358)
Molecular Weight
62,586 Da
3D Structure
ModBase 3D Structure for P51655
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant GPC4 (Thr220-Gly426) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2050360
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-GPC4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-GPC4 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-GPC4 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-GPC4 Polyclonal Antibody – Cat# MBS2001599

Immunohistochemistry (IHC) of anti-GPC4 antibody

DAB staining on fromalin fixed paraffin- embedded stomach tissue)
Rabbit Anti-GPC4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001599

Immunohistochemistry (IHC) of anti-GPC4 antibody

DAB staining on IHC-P; Samples: Mouse Pancreas Tissue)
Rabbit Anti-GPC4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001599

Other Notes

Small volumes of anti-GPC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPC4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glypican-4
NCBI Official Synonym Full Names
glypican 4
NCBI Official Symbol
Gpc4
NCBI Official Synonym Symbols
AF311610; AI385680; AI661372; K-glypican; 9530073D23Rik
UniProt Protein Name
Glypican-4
UniProt Synonym Protein Names
K-glypican
Protein Family
UniProt Gene Name
Gpc4
NCBI Protein Information
glypican-4

UniProt Comments for GPC4

GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: X A5|X 28.33 cM

Cellular Component: external side of plasma membrane; extracellular region; extracellular space; membrane; nucleus; plasma membrane; proteinaceous extracellular matrix

Molecular Function: heparan sulfate proteoglycan binding
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000