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IL-32 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200162

The gene encoding IL32 is located on human chromosome 16p13.3 and is organized into eight exons. IL32 is mainly expressed in natural killer cells, T cells, epithelial cells, and blood monocytes. It can induce the proinflammatory cytokines TNFalpha and IL1beta… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL-32, Monoclonal Antibody
Full Product Name
IL-32 antibody
Product Gene Name
anti-IL-32 antibody
Product Synonym Names
Interleukin 32 isoform A; IL-32; Natural killer cells protein 4 (NK4); Tumor necrosis factor alpha-inducing factor (TAIF); Interleukin 32; Interleukin 32 isoform A IL 32alpha; IL 32beta; IL 32delta; IL 32gamma; Interleukin 32; Natural killer cells protein 4; NK 4; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; il32
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT2F9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
17,003 Da
OMIM
M59807 mRNA
3D Structure
ModBase 3D Structure for P24001
Antigen Species
Human
Immunogen
Recombinant human IL32 (1-131aa) purified from E. coli
Clone Note
Anti-human IL32 mAb, clone AT2F9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL32 protein.

Product Categories/Family for anti-IL-32 antibody

Immunology
Applications Tested/Suitable for anti-IL-32 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-IL-32 antibody
Recommended starting dilution is 1:500

Western Blot (WB) of anti-IL-32 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL32 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: U937 cell lysate Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-32 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-IL-32 Monoclonal Antibody – Cat# MBS200162

Immunofluorescence (IF) of anti-IL-32 antibody

ICC/IF analysis of IL32 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IL32 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-IL-32 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200162

Product References and Citations for anti-IL-32 antibody

Nishida A, et al. (2009) J Biol Chem, 284 (26):17868-76.; Kobayashi H, et al. (2009) Cytokine, 46 (3):351-8.; Mabilleau G, et al. (2009) PLoS One, 4 (1):e4173.

Other Notes

Small volumes of anti-IL-32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-32 purchase

MBS200162 is an antibody targeting IL-32. Also known as IL-32 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-32. It may not necessarily be applicable to this product.

NCBI GI #
61658634
NCBI GeneID
9235
NCBI Accession #
NP_001012651
UniProt Primary Accession #
P24001
UniProt Secondary Accession #
Q5VFH7; Q5VFH8; Q8WV38; Q96GK9; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2
UniProt Related Accession #
P24001
GenBank Nucleotide #
NM_001012633.1
NCBI Official Full Name
interleukin-32 isoform A
NCBI Official Synonym Full Names
interleukin 32
NCBI Official Symbol
IL32
NCBI Official Synonym Symbols
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
UniProt Protein Name
Interleukin-32
UniProt Synonym Protein Names
Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor
UniProt Gene Name
IL32
UniProt Synonym Gene Names
NK4; TAIF; IL-32
NCBI Protein Information
interleukin-32

NCBI Summary for IL-32

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for IL-32

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Research Articles on IL-32

1. In Kaposi sarcoma (KS) splicing ratio of the IL-32 isoforms showed IL-32gamma as the highest expressed isoform, followed by IL-32beta, in HIV-related KS cases compared with controls. Our data suggest a possible survival mechanism by the splicing of IL-32gamma to IL-32beta and also IL-6, IL-8, and CXCR1 signaling pathways to reverse the proapoptotic effect of the IL-32gamma isoform, leading to tumor cell survival and th...
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IL-32 antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Signaling By Interleukins Pathway antibodies  Signaling By Interleukins Pathway Diagram

Diseases associated with anti-IL-32 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >94 publications with IL-32 and Inflammation
 Necrosis Antibodies  >69 publications with IL-32 and Necrosis
 Lung Diseases Antibodies  >25 publications with IL-32 and Lung Diseases
 Nervous System Diseases Antibodies  >11 publications with IL-32 and Nervous System Diseases
 Liver Diseases Antibodies  >11 publications with IL-32 and Liver Diseases
 Cardiovascular Diseases Antibodies  >10 publications with IL-32 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >10 publications with IL-32 and Lung Neoplasms
 Disease Models, Animal Antibodies  >9 publications with IL-32 and Disease Models, Animal
 Stomach Neoplasms Antibodies  >8 publications with IL-32 and Stomach Neoplasms
 Breast Neoplasms Antibodies  >7 publications with IL-32 and Breast Neoplasms

Organs/Tissues associated with anti-IL-32 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >102 publications with IL-32 and Blood
 Lung Antibodies  >27 publications with IL-32 and Lung
 Vascular Antibodies  >20 publications with IL-32 and Vascular
 Bone Antibodies  >15 publications with IL-32 and Bone
 Liver Antibodies  >13 publications with IL-32 and Liver
 Stomach Antibodies  >10 publications with IL-32 and Stomach
 Lymph Node Antibodies  >10 publications with IL-32 and Lymph Node
 Bone Marrow Antibodies  >10 publications with IL-32 and Bone Marrow
 Intestine Antibodies  >10 publications with IL-32 and Intestine
 Muscle Antibodies  >8 publications with IL-32 and Muscle
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