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Neutral Sphingomyelinase (NSMASE) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001629
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MBS2001629 is an antibody targeting NSMASE. Also known as Polyclonal Antibody to Neutral Sphingomyelinase (NSMASE). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Neutral Sphingomyelinase (NSMASE), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Neutral Sphingomyelinase (NSMASE)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.63mg/m (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: NSMASE (MBS2001629)
Biotin Conjugated Antibody: NSMASE (MBS2002988)
Matching Pairs
Unconjugated Antibody: NSMASE (MBS2001629)
Immunogen: NSMASE (MBS2010016)
OMIM
AJ222801 mRNA
3D Structure
ModBase 3D Structure for O60906
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse; Rat
Immunogen
Recombinant NSMASE (Lys2~Arg236) expressed in E.coli (MBS2010016).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NSMASE antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-NSMASE antibody
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-NSMASE antibody

Western Blot:
Sample: Recombinant NSMASE, Human.
Rabbit Anti-NSMASE Polyclonal Antibody – Cat# MBS2001629

Immunohistochemistry (IHC) of anti-NSMASE antibody

DAB staining on IHC-P.
Samples: Human Tissue
Rabbit Anti-NSMASE Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001629

Other Notes

Small volumes of anti-NSMASE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NSMASE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sphingomyelin phosphodiesterase 2
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 2
NCBI Official Symbol
SMPD2
NCBI Official Synonym Symbols
ISC1; NSMASE; NSMASE1
UniProt Protein Name
Sphingomyelin phosphodiesterase 2
UniProt Synonym Protein Names
Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase
Protein Family
UniProt Gene Name
SMPD2
UniProt Synonym Gene Names
Lyso-PAF-PLC; N-SMase; nSMase
NCBI Protein Information
sphingomyelin phosphodiesterase 2

NCBI Summary for NSMASE

This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009]
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UniProt Comments for NSMASE

SMPD2: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. Belongs to the neutral sphingomyelinase family.

Protein type: EC 3.1.4.12; Lipid Metabolism - sphingolipid; Membrane protein, integral; Membrane protein, multi-pass; Phosphodiesterase

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: caveola; integral component of plasma membrane; intracellular; plasma membrane

Molecular Function: metal ion binding; sphingomyelin phosphodiesterase activity

Biological Process: ceramide biosynthetic process; glycosphingolipid metabolic process; response to mechanical stimulus; sphingomyelin metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NSMASE antibody

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