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Keratin 8 (KRT8) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001642
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MBS2001642 is an antibody targeting KRT8. Also known as Polyclonal Antibody to Keratin 8 (KRT8). Reactive in Rat. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Keratin 8 (KRT8), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Keratin 8 (KRT8)
Product Gene Name
anti-KRT8 antibody
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-I AEVRAQYEEI ANRSRAEAET MYQIKYEELQ TLAGKHGDDL RRSKTEISEM NRNISRLQAE IDALKGQRAT LEAAIADAEQ RGELAVKDAN AKLEDLKNAL QKAKQDMARQ LREYQELMNV KLALDIEIAT YRKLLEGE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT8. It has been selected for its ability to recognize KRT8 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: KRT8 (MBS2001642)
Biotin Conjugated Antibody: KRT8 (MBS2006146)
Matching Pairs
Unconjugated Antibody: KRT8 (MBS2001642)
Immunogen: KRT8 (MBS2010436)
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Molecular Weight
54,019 Da
3D Structure
ModBase 3D Structure for Q10758
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant KRT8 (Ile260~Glu398) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062203
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-KRT8 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-KRT8 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-KRT8 antibody

Western Blot: Sample: Human A431 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-KRT8 Polyclonal Antibody – Cat# MBS2001642

Western Blot (WB) of anti-KRT8 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-KRT8 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2001642

Immunohistochemistry (IHC) of anti-KRT8 antibody

DAB staining on IHC-P. Samples: Rat Tissue)
Rabbit Anti-KRT8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001642

Other Notes

Small volumes of anti-KRT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT8. It may not necessarily be applicable to this product.

NCBI GI #
40786432
NCBI GeneID
25626
NCBI Accession #
NP_955402.1
UniProt Primary Accession #
Q10758
UniProt Secondary Accession #
Q5WPB3
UniProt Related Accession #
Q10758
GenBank Nucleotide #
NM_199370.1
NCBI Official Full Name
keratin, type II cytoskeletal 8
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
Krt8
NCBI Official Synonym Symbols
CYKER; Krt2-8
UniProt Protein Name
Keratin, type II cytoskeletal 8
UniProt Synonym Protein Names
Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Protein Family
Keratin
UniProt Gene Name
Krt8
UniProt Synonym Gene Names
Krt2-8; CK-8; K8
NCBI Protein Information
keratin, type II cytoskeletal 8

NCBI Summary for KRT8

an intermediate filament protein; may play a role in prostrate cells [RGD, Feb 2006]

UniProt Comments for KRT8

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: apicolateral plasma membrane; cell periphery; costamere; cytoplasm; dystrophin-associated glycoprotein complex; extracellular exosome; intercellular junction; intermediate filament; intermediate filament cytoskeleton; keratin filament; nuclear matrix; nucleoplasm; nucleus; sarcolemma; Z disc

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: response to hydrostatic pressure; response to other organism; sarcomere organization; tumor necrosis factor-mediated signaling pathway
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Research Articles on KRT8

1. Results indicate a keratins K8/K18 intermediate filament (IF) contribution to the cell stiffness-extracellular matrix (ECM) interplay through a modulation of Rho-dependent actin organization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KRT8 antibody

 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram

Diseases associated with anti-KRT8 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >45 publications with KRT8 and Neoplasms
 Carcinoma Antibodies  >31 publications with KRT8 and Carcinoma
 Liver Diseases Antibodies  >26 publications with KRT8 and Liver Diseases
 Gastrointestinal Diseases Antibodies  >14 publications with KRT8 and Gastrointestinal Diseases
 Adenocarcinoma Antibodies  >14 publications with KRT8 and Adenocarcinoma
 Hepatitis Antibodies  >11 publications with KRT8 and Hepatitis
 Breast Neoplasms Antibodies  >10 publications with KRT8 and Breast Neoplasms
 Liver Cirrhosis Antibodies  >9 publications with KRT8 and Liver Cirrhosis
 Neoplasm Metastasis Antibodies  >9 publications with KRT8 and Neoplasm Metastasis
 Fibrosis Antibodies  >9 publications with KRT8 and Fibrosis

Organs/Tissues associated with anti-KRT8 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >36 publications with KRT8 and Liver
 Embryonic Tissue Antibodies  >12 publications with KRT8 and Embryonic Tissue
 Kidney Antibodies  >8 publications with KRT8 and Kidney
 Lung Antibodies  >6 publications with KRT8 and Lung
 Thymus Antibodies  >5 publications with KRT8 and Thymus
 Colon Antibodies  >5 publications with KRT8 and Colon
 Eye Antibodies  >5 publications with KRT8 and Eye
 Prostate Antibodies  >4 publications with KRT8 and Prostate
 Pancreas Antibodies  >4 publications with KRT8 and Pancreas
 Placenta Antibodies  >2 publications with KRT8 and Placenta
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