Mouse Anti-AGXT Monoclonal Antibody – Cat# MBS200170

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-AGXT antibody (1:500) with Alexa 488 (Green). ICC/IF analysis of AGXT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AGXT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

AGXT Antibody – Mouse Monoclonal

Name: AGXT Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS200170
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200170

The AGXT gene provides instructions for making a liver enzyme called alanine-glyoxylate aminotransferase. Inside liver cells, this enzyme is found in peroxisomes, structures that contain many different enzymes used to produce energy and the basic materials… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

AGXT, Monoclonal Antibody

Full Product Name

AGXT antibody

Product Gene Name

anti-AGXT antibody

Product Synonym Names

Serpin B5; Protease inhibitor 5; SERPINB5; alanine-glyoxylate aminotransferase; AGXT1; PH1; AGT; SPT; AGT1; oxalosis I; primary hyperoxaluria type 1; L-alanine: glyoxylate aminotransferase 1; serine:pyruvate aminotransferase; glycolicaciduria

Clonality

Monoclonal

Isotype

IgG2b, k

Clone Number

AT2T4

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

Purity/Purification

By protein-G affinity chromatography

Concentration

1mg/ml (lot specific)

Preparation and Storage

Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

43,010 Da

OMIM

X56092 mRNA

3D Structure

ModBase 3D Structure for P21549

Antigen Species

Human

Immunogen

Recombinant AGXT (330-392aa) purified from E. coli

Clone Note

Anti-human AGXT mAb, clone AT2T4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AGXT protein.

Product Categories/Family for anti-AGXT antibody

Metabolism

Applications Tested/Suitable for anti-AGXT antibody

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Application Notes for anti-AGXT antibody

IF: 1:500 ~ 1000.
Recommended starting dilution is 1:500.

Product References and Citations for anti-AGXT antibody

Donini S, et al., (2009) Biochem J 422 (2):265-272 Grujic D, et al., (2009) Am J Nephrol 29 (2):86-93 Williams EL, et al., (2009) Hum Mutat 30 (6):910-917

Other Notes

Small volumes of anti-AGXT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AGXT purchase

MBS200170 is an antibody targeting AGXT. Also known as AGXT antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for AGXT. It may not necessarily be applicable to this product.

NCBI GI #

4557289

NCBI GeneID

189

NCBI Accession #

NP_000021

UniProt Primary Accession #

P21549

UniProt Secondary Accession #

Q53QU6

UniProt Related Accession #

P21549

GenBank Nucleotide #

NM_000030.2

NCBI Official Full Name

serine--pyruvate aminotransferase

NCBI Official Synonym Full Names

alanine-glyoxylate aminotransferase

NCBI Official Symbol

AGXT

NCBI Official Synonym Symbols

AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1

UniProt Protein Name

Serine--pyruvate aminotransferase

UniProt Synonym Protein Names

Alanine--glyoxylate aminotransferase (EC:2.6.1.44); AGT

Protein Family

Serine--pyruvate aminotransferase

UniProt Gene Name

AGXT

UniProt Synonym Gene Names

AGT1; SPAT; SPT; AGT

NCBI Protein Information

serine--pyruvate aminotransferase

NCBI Summary for AGXT

This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]

Research Articles on AGXT

1. this study identifies a novel AGXT gene mutation in primary hyperoxaluria after kidney transplantation failure in Tunisian patient

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AGXT antibody

Reference Product	PubMed Publications
GRHPR antibody	>17 publications with AGXT and GRHPR
HOGA1 antibody	>8 publications with AGXT and HOGA1
GLDC antibody	>1 publications with AGXT and GLDC
AGXT2 antibody	>1 publications with AGXT and AGXT2

Pathways associated with anti-AGXT antibody

Products by Pathway	Pathway Diagram
Alanine And Aspartate Metabolism Pathway antibodies	Alanine And Aspartate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Glyoxylate Metabolism And Glycine Degradation Pathway antibodies	Glyoxylate Metabolism And Glycine Degradation Pathway Diagram

Diseases associated with anti-AGXT antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>91 publications with AGXT and Kidney Diseases
Primary hyperoxaluria type 1 Antibodies	>77 publications with AGXT and Primary hyperoxaluria type 1
Liver Diseases Antibodies	>10 publications with AGXT and Liver Diseases
Disease Models, Animal Antibodies	>6 publications with AGXT and Disease Models, Animal
Neoplasms, Experimental Antibodies	>3 publications with AGXT and Neoplasms, Experimental
Hypertension Antibodies	>3 publications with AGXT and Hypertension
Poisoning Antibodies	>2 publications with AGXT and Poisoning
Fatty Liver Antibodies	>2 publications with AGXT and Fatty Liver
Cardiovascular Diseases Antibodies	>2 publications with AGXT and Cardiovascular Diseases
Breast Neoplasms Antibodies	>1 publications with AGXT and Breast Neoplasms

Organs/Tissues associated with anti-AGXT antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>56 publications with AGXT and Liver
Kidney Antibodies	>52 publications with AGXT and Kidney
Blood Antibodies	>13 publications with AGXT and Blood
Intestine Antibodies	>2 publications with AGXT and Intestine
Heart Antibodies	>2 publications with AGXT and Heart
Brain Antibodies	>2 publications with AGXT and Brain