• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TYMS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200172

The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dMTP) is an essential step for the formation of thymine nucleotides. This process is catalyzed by thymidylate synthase (TS or TYMS). TYMS is an intracellular enzyme that… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TYMS, Monoclonal Antibody
Full Product Name
TYMS antibody
Product Gene Name
anti-TYMS antibody
Product Synonym Names
HST422; TMS; TS; d TMP synthase; EC 2.1.1.45; MGC88736; Thymidylate synthase; Thymidylate synthetase; Tsase; TYMS; TYMS protein; Tyms thymidylate synthetase
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT21S5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
26,140 Da
OMIM
X02308 mRNA
3D Structure
ModBase 3D Structure for P04818
Antigen Species
Human
Immunogen
Recombinant TYMS (1-313aa) purified from E. coli
Clone Note
Anti-human TYMS mAb, clone AT1S5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TYMS protein.

Product Categories/Family for anti-TYMS antibody

Cancer
Applications Tested/Suitable for anti-TYMS antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-TYMS antibody
WB & IF: 1:500 ~ 1000.
Recommended starting dilution is 1:500.

Western Blot (WB) of anti-TYMS antibody

The recombinant human TYMS protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TYMS (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: MCF7 cell lysate
Mouse Anti-TYMS Monoclonal Antibody – Cat# MBS200172

Immunofluorescence (IF) of anti-TYMS antibody

HeLa cells were stained with monoclonal anti-TYMS antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue). ICC/IF analysis of TYMS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TYMS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-TYMS Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200172

Product References and Citations for anti-TYMS antibody

Shahrokni A, et al., (2009) Clin Colorectal Cancer 8 (4):231-4; Henriquez-Hernandez LA, et al., (2009) Oncol Rep 22 (6):1425-33; Colavito D, et al., (2009) Cancer Chemother Pharmacol 64 (6):1195-200

Other Notes

Small volumes of anti-TYMS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TYMS purchase

MBS200172 is an antibody targeting TYMS. Also known as TYMS antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TYMS. It may not necessarily be applicable to this product.

NCBI GI #
4507751
NCBI GeneID
7298
NCBI Accession #
NP_001062
UniProt Primary Accession #
P04818
UniProt Secondary Accession #
Q8WYK3; Q8WYK4
UniProt Related Accession #
P04818
GenBank Nucleotide #
NM_001071.2
NCBI Official Full Name
thymidylate synthase
NCBI Official Synonym Full Names
thymidylate synthetase
NCBI Official Symbol
TYMS
NCBI Official Synonym Symbols
TS; TMS; HST422
UniProt Protein Name
Thymidylate synthase
Protein Family
TYMS opposite strand protein
UniProt Gene Name
TYMS
UniProt Synonym Gene Names
TS; TS; TSase
NCBI Protein Information
thymidylate synthase

NCBI Summary for TYMS

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]
Show more

UniProt Comments for TYMS

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Research Articles on TYMS

1. High expression level of TYMS is associated with neuroendocrine lung tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TYMS antibody

 Reference Product  PubMed Publications
 DHFR antibody  >48 publications with TYMS and DHFR
 SHMT1 antibody  >32 publications with TYMS and SHMT1
 MTHFD1 antibody  >28 publications with TYMS and MTHFD1
 FPGS antibody  >15 publications with TYMS and FPGS
 TK1 antibody  >7 publications with TYMS and TK1
 DTYMK antibody  >3 publications with TYMS and DTYMK
 RRM2 antibody  >2 publications with TYMS and RRM2
 DCTD antibody  >2 publications with TYMS and DCTD
 DHFRL1 antibody  >1 publications with TYMS and DHFRL1
 DUT antibody  >1 publications with TYMS and DUT

Pathways associated with anti-TYMS antibody

 Products by Pathway  Pathway Diagram
 Antifolate Resistance Pathway antibodies  Antifolate Resistance Pathway Diagram
 Antifolate Resistance Pathway antibodies  Antifolate Resistance Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Circadian Rythm Related Genes Pathway antibodies  Circadian Rythm Related Genes Pathway Diagram
 E2F Mediated Regulation Of DNA Replication Pathway antibodies  E2F Mediated Regulation Of DNA Replication Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Fluoropyrimidine Activity Pathway antibodies  Fluoropyrimidine Activity Pathway Diagram
 G1/S Transition Pathway antibodies  G1/S Transition Pathway Diagram
 G1/S-Specific Transcription Pathway antibodies  G1/S-Specific Transcription Pathway Diagram

Diseases associated with anti-TYMS antibody

 Disease Name  Pubmed Publications
 Digestive System Neoplasms Antibodies  >121 publications with TYMS and Digestive System Neoplasms
 Colorectal Neoplasms Antibodies  >87 publications with TYMS and Colorectal Neoplasms
 Adenocarcinoma Antibodies  >44 publications with TYMS and Adenocarcinoma
 Breast Neoplasms Antibodies  >42 publications with TYMS and Breast Neoplasms
 Lung Neoplasms Antibodies  >39 publications with TYMS and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >26 publications with TYMS and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >23 publications with TYMS and Neoplasms, Experimental
 Head and Neck Neoplasms Antibodies  >22 publications with TYMS and Head and Neck Neoplasms
 Diarrhea Antibodies  >19 publications with TYMS and Diarrhea
 Stomach Neoplasms Antibodies  >18 publications with TYMS and Stomach Neoplasms

Organs/Tissues associated with anti-TYMS antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >92 publications with TYMS and Blood
 Lung Antibodies  >50 publications with TYMS and Lung
 Stomach Antibodies  >18 publications with TYMS and Stomach
 Liver Antibodies  >12 publications with TYMS and Liver
 Vascular Antibodies  >10 publications with TYMS and Vascular
 Heart Antibodies  >9 publications with TYMS and Heart
 Pancreas Antibodies  >8 publications with TYMS and Pancreas
 Lymph Antibodies  >7 publications with TYMS and Lymph
 Lymph Node Antibodies  >7 publications with TYMS and Lymph Node
 Bladder Antibodies  >6 publications with TYMS and Bladder
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000