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KRT18 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200177

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8)… More details

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Product Overview

Online Datasheet in PDF
Product Name
KRT18, Monoclonal Antibody
Full Product Name
KRT18 antibody
Product Gene Name
Product Synonym Names
Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; CK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; K18; Keratin 18; Keratin D; Keratin type I cytoskeletal 18; Kerd
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT2G3
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
48,058 Da
OMIM
X12881 mRNA
3D Structure
ModBase 3D Structure for P05783
Antigen Species
Human
Immunogen
Recombinant human KRT18 (79-430aa) purified from E. coli
Clone Note
Anti-human KRT18 mAb, clone AT2G3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT18 protein.

Product Categories/Family for anti-KRT18 antibody

Applications Tested/Suitable for anti-KRT18 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-KRT18 antibody
WB/IF: 1:250 ~ 1000.
Recommended starting dilution is 1:500

Western Blot (WB) of anti-KRT18 antibody

The Cell lysates of HeLa and AGS (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate
Mouse Anti-KRT18 Monoclonal Antibody – Cat# MBS200177

Immunohistochemistry (IHC) of anti-KRT18 antibody

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human KRT18 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Mouse Anti-KRT18 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200177

Immunofluorescence (IF) of anti-KRT18 antibody

Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:500) with Texas Red (Red). ICC/IF analysis of KRT18 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-KRT18 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200177

Product References and Citations for anti-KRT18 antibody

Moll, R, et al,. (1988) Am. J. Pathol., 132: 123-44; Schlimok, G, et al,. (1988) Arzneimittel-Forschung, 38: 435-7; Debus, E, et al,. (1984) Am. J. Pathol., 114: 121-30 (1984)

Other Notes

Small volumes of anti-KRT18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT18 purchase

MBS200177 is an antibody targeting KRT18. Also known as KRT18 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT18. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Synonym Protein Names
Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Protein Family
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
NCBI Protein Information
keratin, type I cytoskeletal 18

NCBI Summary for KRT18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT18

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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