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SUMO2/3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200184

SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8-11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to… More details

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Product Overview

Online Datasheet in PDF
Product Name
SUMO2/3, Monoclonal Antibody
Full Product Name
SUMO2/3 antibody
Product Gene Name
anti-SUMO2/3 antibody
Product Synonym Names
Small ubiquitin-related modifier 2; SuMO-2; SuMO-3; Sentrin-2; HSMT3; SMT3B; SMT3H2; Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S; cerevisiae); HSMT 3; MGC117191; Sentrin 2; Sentrin2; Small ubiquitin like modifier 2; Small ubiquitin related modifier 2; SMT 3B; SMT3 homolog 2; SMT3 suppressor of mif two 3 homolog 2; Sumo2; Sumo3; ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3; SMT3A; SMT3H1; SuMO-3; sumo3; sumo 3
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT10F1
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at +2°C to +8°C for one week. For long term storage , aliqout and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
OMIM
L76416 mRNA
3D Structure
ModBase 3D Structure for P61956
Antigen Species
Human
Immunogen
Recombinant human SUMO2 (1-93aa) purified from E Coli
Clone Note
Anti-human SUMO2/3 mAb, clone AT10F1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SUMO2 protein.

Product Categories/Family for anti-SUMO2/3 antibody

Cancer
Applications Tested/Suitable for anti-SUMO2/3 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC)
Application Notes for anti-SUMO2/3 antibody
The antibody has been tested by ELISA, Western Blot, ICC/IF, FC, and IHC analysis to assure specificity and reactivity . Since application varies , however , each other investigation should be titrated by the reagent to obtain optimal reuslts.

Western Blot (WB) of anti-SUMO2/3 antibody

1ST WB: The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: K562 cell lysate

2ND WB:The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HL-60 cell lysate

3RD WB: The recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human SUMO1 proteinLane 2.: Recombinant human SUMO2 protein Lane 3.: Recombinant human SUMO3 protein
Mouse Anti-SUMO2/3 Monoclonal Antibody – Cat# MBS200184

Immunohistochemistry (IHC) of anti-SUMO2/3 antibody

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Mouse Anti-SUMO2/3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200184

Immunofluorescence (IF) of anti-SUMO2/3 antibody

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red).
Mouse Anti-SUMO2/3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200184

Flow Cytometry (FC) of anti-SUMO2/3 antibody

Flow cytometry analysis of SUMO2/3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Mouse Anti-SUMO2/3 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS200184

Product References and Citations for anti-SUMO2/3 antibody

Tatsuya Ii, et al. (2007) DNA Repair (Amst), 6 (11): 1679-1691.; Zheng Xu, et al. (2005) Biochem J, 386 (Pt 2): 325-330.; Melchior F. (2000) Annu Rev Cell Dev Biol, 16: 591-626.

Other Notes

Small volumes of anti-SUMO2/3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SUMO2/3 purchase

MBS200184 is an antibody targeting SUMO2/3. Also known as SUMO2/3 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SUMO2/3. It may not necessarily be applicable to this product.

NCBI GI #
54792069
NCBI GeneID
6613
NCBI Accession #
NP_008868
UniProt Primary Accession #
P61956
UniProt Secondary Accession #
P55855; Q32Q42; Q6IPZ6; Q96HK1; B2R4I2
UniProt Related Accession #
P61956
GenBank Nucleotide #
NM_006937.3
NCBI Official Full Name
small ubiquitin-related modifier 2 isoform a
NCBI Official Synonym Full Names
small ubiquitin-like modifier 2
NCBI Official Symbol
SUMO2
NCBI Official Synonym Symbols
HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
UniProt Protein Name
Small ubiquitin-related modifier 2
UniProt Synonym Protein Names
HSMT3
UniProt Gene Name
SUMO2
UniProt Synonym Gene Names
Smt3B
NCBI Protein Information
small ubiquitin-related modifier 2

NCBI Summary for SUMO2/3

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for SUMO2/3

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).
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Research Articles on SUMO2/3

1. We further show that the SUMO2 pathway acts independently of exogenous C-MYC expression and in parallel with small-molecule enhancers of reprogramming. Importantly, suppression of SUMO2 also promotes the generation of human induced pluripotent stem cells .

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SUMO2/3 antibody

 Reference Product  PubMed Publications
 SENP1 antibody  >25 publications with SUMO2/3 and SENP1
 PIAS1 antibody  >16 publications with SUMO2/3 and PIAS1
 RANGAP1 antibody  >16 publications with SUMO2/3 and RANGAP1
 SENP2 antibody  >16 publications with SUMO2/3 and SENP2
 PIAS4 antibody  >13 publications with SUMO2/3 and PIAS4
 RANBP2 antibody  >10 publications with SUMO2/3 and RANBP2
 UBA2 antibody  >8 publications with SUMO2/3 and UBA2
 SAE1 antibody  >5 publications with SUMO2/3 and SAE1
 UBE2I antibody  >5 publications with SUMO2/3 and UBE2I
 SENP5 antibody  >4 publications with SUMO2/3 and SENP5

Pathways associated with anti-SUMO2/3 antibody

 Products by Pathway  Pathway Diagram
 DNA Double-Strand Break Repair Pathway antibodies  DNA Double-Strand Break Repair Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Fluid Shear Stress And Atherosclerosis Pathway antibodies  Fluid Shear Stress And Atherosclerosis Pathway Diagram
 Fluid Shear Stress And Atherosclerosis Pathway antibodies  Fluid Shear Stress And Atherosclerosis Pathway Diagram
 Formation Of Incision Complex In GG-NER Pathway antibodies  Formation Of Incision Complex In GG-NER Pathway Diagram
 Global Genome Nucleotide Excision Repair (GG-NER) Pathway antibodies  Global Genome Nucleotide Excision Repair (GG-NER) Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 HDR Through Homologous Recombination (HR) Or Single Strand Annealing (SSA) Pathway antibodies  HDR Through Homologous Recombination (HR) Or Single Strand Annealing (SSA) Pathway Diagram
 Homology Directed Repair Pathway antibodies  Homology Directed Repair Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram

Diseases associated with anti-SUMO2/3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >13 publications with SUMO2/3 and Inflammation
 Carcinoma Antibodies  >10 publications with SUMO2/3 and Carcinoma
 Immune System Diseases Antibodies  >8 publications with SUMO2/3 and Immune System Diseases
 Necrosis Antibodies  >8 publications with SUMO2/3 and Necrosis
 Skin Diseases Antibodies  >7 publications with SUMO2/3 and Skin Diseases
 Liver Diseases Antibodies  >7 publications with SUMO2/3 and Liver Diseases
 Kidney Diseases Antibodies  >5 publications with SUMO2/3 and Kidney Diseases
 Cell Transformation, Neoplastic Antibodies  >4 publications with SUMO2/3 and Cell Transformation, Neoplastic
 Heart Diseases Antibodies  >4 publications with SUMO2/3 and Heart Diseases
 Fibrosis Antibodies  >3 publications with SUMO2/3 and Fibrosis

Organs/Tissues associated with anti-SUMO2/3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >28 publications with SUMO2/3 and Brain
 Blood Antibodies  >23 publications with SUMO2/3 and Blood
 Liver Antibodies  >17 publications with SUMO2/3 and Liver
 Kidney Antibodies  >12 publications with SUMO2/3 and Kidney
 Heart Antibodies  >11 publications with SUMO2/3 and Heart
 Prostate Antibodies  >8 publications with SUMO2/3 and Prostate
 Skin Antibodies  >6 publications with SUMO2/3 and Skin
 Testis Antibodies  >5 publications with SUMO2/3 and Testis
 Lung Antibodies  >4 publications with SUMO2/3 and Lung
 Embryonic Tissue Antibodies  >4 publications with SUMO2/3 and Embryonic Tissue
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