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Interleukin 3 Receptor Alpha (IL3Ra) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001865
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MBS2001865 is an antibody targeting IL3Ra. Also known as Polyclonal Antibody to Interleukin 3 Receptor Alpha (IL3Ra). Reactive in Human, Rat. Host: Rabbit. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin 3 Receptor Alpha (IL3Ra), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Interleukin 3 Receptor Alpha (IL3Ra)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-TK EGKPWAGAEN LTCWIHDVDF LSCSWAVGPG APADVQYDLY LNVANRRQQY ECLHYKTDAQ GTRIGCRFDD ISRLSSGSQS SHILVRGRSA AFGIPCTDKF VVFSQIEILT PPNMTAKCNK THSFMHWKMR SHFNRKF
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against IL3Ra. It has been selected for its ability to recognize IL3Ra in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: IL3Ra (MBS2001865)
Biotin Conjugated Antibody: IL3Ra (MBS2001643)
Matching Pairs
Unconjugated Antibody: IL3Ra (MBS2001865)
Immunogen: IL3Ra (MBS2009126)
Molecular Weight
34,466 Da
OMIM
M74782 mRNA
3D Structure
ModBase 3D Structure for P26951
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant IL3Ra (Thr19~Phe157) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2056021
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-IL3Ra antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL3Ra antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-IL3Ra antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-IL3Ra Polyclonal Antibody – Cat# MBS2001865

Immunohistochemistry (IHC) of anti-IL3Ra antibody

DAB staining on fromalin fixed paraffin- embedded kidney tissue)
Rabbit Anti-IL3Ra Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001865

Immunohistochemistry (IHC) of anti-IL3Ra antibody

DAB staining on IHC-P; Samples: Human Kidney Tissue)
Rabbit Anti-IL3Ra Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001865

Other Notes

Small volumes of anti-IL3Ra antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL3Ra. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-3 receptor subunit alpha isoform 2
NCBI Official Synonym Full Names
interleukin 3 receptor subunit alpha
NCBI Official Symbol
IL3RA
NCBI Official Synonym Symbols
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
UniProt Protein Name
Interleukin-3 receptor subunit alpha
UniProt Synonym Protein Names
CD_antigen: CD123
UniProt Gene Name
IL3RA
UniProt Synonym Gene Names
IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
NCBI Protein Information
interleukin-3 receptor subunit alpha

NCBI Summary for IL3Ra

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
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UniProt Comments for IL3Ra

IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.33 and Yp11.2

Cellular Component: integral component of membrane; intracellular; plasma membrane

Molecular Function: interleukin-3 receptor activity; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: MAPK cascade; peptidyl-tyrosine phosphorylation; positive regulation of GTPase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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