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NQO2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200187

Quinone oxidoreductase (NQO1 and NQO2) are cytosolic proteins that catalyze metabolic redaction of quinines and derivates.NQO2 is inhibited by flavones such as quercetin. Also benzo (a)pyrene is another known inhibitor of NQO2. Even though overlapping… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NQO2, Monoclonal Antibody
Full Product Name
NQO2 antibody
Product Gene Name
Product Synonym Names
Ribosyldihydronicotinamide dehydrogenase; DHQV; DIA6; QR2; Ribosyldihydronicotinamide dehydrogenase Ox2; EC 1.10.99.2; MGC94180; NAD (P)H dehydrogenase quinone 2; NAD (P)H menadione oxidoreductase 1 dioxin inducible 2; NAD (P)H menadione oxidoreductase 2 dioxin inducible; NMOR2; NQO 2; NRH dehydrogenase; NRH:quinone oxidoreductase 2; OTTHuMP00000015948; OTTHuMP00000015949; OTTHuMP00000015953; Ox 2; QR2; Quinone; Quinone reductase 2; Ribosyldihydronicotinamide dehydrogenase.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT1E3
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
25,919 Da
OMIM
J02888 mRNA
Antigen Species
Human
Immunogen
Recombinant human NQO2 (1-231aa) purified from E. coli
Clone Note
Anti-human NQO2 mAb, clone AT1E3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NQO2 protein.

Product Categories/Family for anti-NQO2 antibody

Applications Tested/Suitable for anti-NQO2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NQO2 antibody
WB: 1:250 ~ 500.
Recommended starting dilution is 1:500

Western Blot (WB) of anti-NQO2 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: A549 cell lysate
Mouse Anti-NQO2 Monoclonal Antibody – Cat# MBS200187

Immunofluorescence (IF) of anti-NQO2 antibody

ICC/IF analysis of NQO2 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NQO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-NQO2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200187

Product References and Citations for anti-NQO2 antibody

Wu K., et al,. (1997) Arch Biochem Biophys. 347 (2):221-8.; Aiswal AK., et al,. (1994) J Biol Chem. 269 (20):14502-8.

Other Notes

Small volumes of anti-NQO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NQO2 purchase

MBS200187 is an antibody targeting NQO2. Also known as NQO2 antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NQO2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
NAD(P)H dehydrogenase, quinone 2
NCBI Official Synonym Full Names
N-ribosyldihydronicotinamide:quinone reductase 2
NCBI Official Symbol
NQO2
NCBI Official Synonym Symbols
QR2; DHQV; DIA6; NMOR2
UniProt Protein Name
Ribosyldihydronicotinamide dehydrogenase [quinone]
UniProt Synonym Protein Names
NRH dehydrogenase [quinone] 2; NRH:quinone oxidoreductase 2; Quinone reductase 2; QR2
UniProt Gene Name
NQO2
UniProt Synonym Gene Names
NMOR2; QR2
NCBI Protein Information
ribosyldihydronicotinamide dehydrogenase [quinone]

NCBI Summary for NQO2

This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
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UniProt Comments for NQO2

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NQO2 antibody

 Reference Product  PubMed Publications
 AKT1 antibody  >2 publications with NQO2 and AKT1
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