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BCL-2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200190

Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Bcl-2, Monoclonal Antibody
Full Product Name
Bcl-2 antibody
Product Gene Name
Product Synonym Names
B-cell lymphoma protein 2 alpha; B-cell lymphoma protein 2 alpha; B-cell lymphoma protein 2 alpha Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; Bcl-2; Bcl2; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma; B-cell; 2; Oncogene B-cell leukemia 2.
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1B5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
22,337 Da
OMIM
M13994 mRNA
3D Structure
ModBase 3D Structure for P10415
Antigen Species
Human
Immunogen
Recombinant human Bcl-2 (1-211aa) purified from E. coli
Clone Note
Anti-human Bcl2 mAb, clone AT1B5, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Bcl-2 protein.

Product Categories/Family for anti-Bcl-2 antibody

Applications Tested/Suitable for anti-Bcl-2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-Bcl-2 antibody
The antibody has been tested by ELISA, Western blot, and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results.
Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.
Recommended starting dilution is 1:500

Western Blot (WB) of anti-Bcl-2 antibody

Cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-BCL-2 Monoclonal Antibody – Cat# MBS200190

Immunofluorescence (IF) of anti-Bcl-2 antibody

Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with monoclonal anti-human Bcl-2 antibody (1:500) with Texas Red (Red).
Mouse Anti-BCL-2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200190

Product References and Citations for anti-Bcl-2 antibody

Zamzami N., et al,. (1998) Oncogene. 16 (17):2265-82.; Fernandez-Sarabia MJ., et al,. (1993) Nature. 366 (6452):274-5.

Other Notes

Small volumes of anti-Bcl-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bcl-2 purchase

MBS200190 is an antibody targeting Bcl-2. Also known as Bcl-2 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Bcl-2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apoptosis regulator Bcl-2 alpha isoform
NCBI Official Synonym Full Names
BCL2, apoptosis regulator
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
NCBI Protein Information
apoptosis regulator Bcl-2

NCBI Summary for Bcl-2

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
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UniProt Comments for Bcl-2

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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