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MINCLE Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200192

MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MINCLE, Monoclonal Antibody
Full Product Name
MINCLE antibody
Product Gene Name
anti-MINCLE antibody
Product Synonym Names
C-type lectin domain family 4 member E; CLECSF9; C-type lectin superfamily member 9; Macrophage-inducible C-type lectin; CLEC 4E; CLEC4E
Clonality
Monoclonal
Isotype
IgG2b, κ
Clone Number
AT16E3
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2-8°C for one week
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
OMIM
AB024718 mRNA
3D Structure
ModBase 3D Structure for Q9ULY5
Immunogen
Recombinant human MINCLE (41-219aa) purified from E Coli

Product Categories/Family for anti-MINCLE antibody

Immunology
Applications Tested/Suitable for anti-MINCLE antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-MINCLE antibody
This antibody has been tested by ELISA, Western Blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since applications varies, however, each investigation should be titrated by the reagent to obtain optimal results

Western Blot (WB) of anti-MINCLE antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MINCLE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : 293T cell lysate
Lane 2. : MINCLE transfected 293T cell lysate
Mouse Anti-MINCLE Monoclonal Antibody – Cat# MBS200192

Immunohistochemistry (IHC) of anti-MINCLE antibody

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human MINCLE antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Mouse Anti-MINCLE Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200192

Immunofluorescence (IF) of anti-MINCLE antibody

Immunofluorescence of human HeLa cells stained with monoclonal anti-human MINCLE antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Mouse Anti-MINCLE Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200192

Flow Cytometry (FC) of anti-MINCLE antibody

Flow cytometry analysis of CLEC4E in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line).
The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Isotype control antibody was mouse IgG (black line).
Mouse Anti-MINCLE Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS200192

Product References and Citations for anti-MINCLE antibody

Ishikawa E, et al. (2009) J Exp Med, 206 (13):2879-88; Matsunaga I, et al. (2009) J Exp Med, 206 (13):2865-8.; Graham LM, et al. (2009) Cytokine, 48 (1-2):148-55.

Other Notes

Small volumes of anti-MINCLE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MINCLE purchase

MBS200192 is an antibody targeting MINCLE. Also known as MINCLE antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MINCLE. It may not necessarily be applicable to this product.

NCBI GI #
7657333
NCBI GeneID
26253
NCBI Accession #
NP_055173
UniProt Primary Accession #
Q9ULY5
UniProt Secondary Accession #
B2R6Q6
UniProt Related Accession #
Q9ULY5
GenBank Nucleotide #
NM_014358.3
NCBI Official Full Name
C-type lectin domain family 4 member E
NCBI Official Synonym Full Names
C-type lectin domain family 4 member E
NCBI Official Symbol
CLEC4E
NCBI Official Synonym Symbols
MINCLE; CLECSF9
UniProt Protein Name
C-type lectin domain family 4 member E
UniProt Synonym Protein Names
C-type lectin superfamily member 9; Macrophage-inducible C-type lectin
UniProt Gene Name
CLEC4E
UniProt Synonym Gene Names
CLECSF9; MINCLE
NCBI Protein Information
C-type lectin domain family 4 member E

NCBI Summary for MINCLE

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
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UniProt Comments for MINCLE

C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.
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Research Articles on MINCLE

1. No association was detected with any of the SNPs analysed and susceptibility to tuberculosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MINCLE antibody

 Reference Product  PubMed Publications
 SYK antibody  >4 publications with MINCLE and SYK
 CARD9 antibody  >3 publications with MINCLE and CARD9
 TLR2 antibody  >3 publications with MINCLE and TLR2

Pathways associated with anti-MINCLE antibody

 Products by Pathway  Pathway Diagram
 C-type Lectin Receptors (CLRs) Pathway antibodies  C-type Lectin Receptors (CLRs) Pathway Diagram
 Dectin-2 Family Pathway antibodies  Dectin-2 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram

Diseases associated with anti-MINCLE antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >14 publications with MINCLE and Inflammation
 Necrosis Antibodies  >4 publications with MINCLE and Necrosis
 Cardiovascular Diseases Antibodies  >4 publications with MINCLE and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >3 publications with MINCLE and Disease Models, Animal
 Lung Diseases Antibodies  >3 publications with MINCLE and Lung Diseases
 Kidney Diseases Antibodies  >2 publications with MINCLE and Kidney Diseases
 Atherosclerosis Antibodies  >2 publications with MINCLE and Atherosclerosis
 Liver Diseases Antibodies  >2 publications with MINCLE and Liver Diseases
 Brain Injuries Antibodies  >2 publications with MINCLE and Brain Injuries
 Lung Injury Antibodies  >1 publications with MINCLE and Lung Injury

Organs/Tissues associated with anti-MINCLE antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with MINCLE and Blood
 Spleen Antibodies  >4 publications with MINCLE and Spleen
 Lung Antibodies  >4 publications with MINCLE and Lung
 Kidney Antibodies  >3 publications with MINCLE and Kidney
 Brain Antibodies  >2 publications with MINCLE and Brain
 Mixed Antibodies  >1 publications with MINCLE and Mixed
 Connective Tissue Antibodies  >1 publications with MINCLE and Connective Tissue
 Intestine Antibodies  >1 publications with MINCLE and Intestine
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