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PKLR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200204

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the… More details

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Product Overview

Online Datasheet in PDF
Product Name
PKLR, Monoclonal Antibody
Full Product Name
PKLR antibody
Product Gene Name
Product Synonym Names
Pyruvate kinase isozymes R/L; PK1; PKL; PKR; PKRL; RPK
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT1E3
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
58,494 Da
OMIM
AB015983 mRNA
3D Structure
ModBase 3D Structure for P30613
Antigen Species
Human
Immunogen
Recombinant human PKLR (47-574aa) purified from E. coli
Clone Note
Anti-human PKLR mAb, clone AT1E3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PKLR protein.

Product Categories/Family for anti-PKLR antibody

Applications Tested/Suitable for anti-PKLR antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PKLR antibody
WB: 1:500

Western Blot (WB) of anti-PKLR antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Balb-3T3 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Raji cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate
Mouse Anti-PKLR Monoclonal Antibody – Cat# MBS200204

Immunofluorescence (IF) of anti-PKLR antibody

ICC/IF analysis of PKLR in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKLR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PKLR in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKLR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PKLR Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200204

Product References and Citations for anti-PKLR antibody

Meza NW., et al. (2009). Mol Ther. 17 (12):2000-9.; Roy MF., et al. (2007). J Exp Med. 204 (12):2949-61.

Other Notes

Small volumes of anti-PKLR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKLR purchase

MBS200204 is an antibody targeting PKLR. Also known as PKLR antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PKLR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pyruvate kinase PKLR isoform 1
NCBI Official Synonym Full Names
pyruvate kinase L/R
NCBI Official Symbol
PKLR
NCBI Official Synonym Symbols
PK1; PKL; PKR; RPK; PKRL
UniProt Protein Name
Pyruvate kinase PKLR
UniProt Synonym Protein Names
Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
Protein Family
UniProt Gene Name
PKLR
UniProt Synonym Gene Names
PK1; PKL
NCBI Protein Information
pyruvate kinase PKLR

NCBI Summary for PKLR

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for PKLR

Plays a key role in glycolysis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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