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RAS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200206

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP,… More details

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Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RAS, Monoclonal Antibody
Full Product Name
RAS antibody
Product Gene Name
anti-RAS antibody
Product Synonym Names
Neuroblastoma RAS viral (v-ras) oncogene homolog; GTPase NRas; HRAS1; ALPS4; N-ras; NRAS1; NS6; Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521; AV095280; N ras; N ras protein part 4; Neuroblastoma RAS viral (v ras) oncogene homolog; OTTHuMP00000013879; Transforming protein N Ras; v ras neuroblastoma RAS viral oncogene homolog
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT2G9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
21,229 Da
OMIM
X02751 mRNA
3D Structure
ModBase 3D Structure for P01111
Antigen Species
Human
Immunogen
Recombinant human NRAS (1-186aa) purified from E. coli
Clone Note
Anti-human RAS mAb, clone AT2G9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NRAS protein.

Product Categories/Family for anti-RAS antibody

Signal Transduction
Applications Tested/Suitable for anti-RAS antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-RAS antibody
WB: 1:500 ~ 1:5000.
Recommended starting dilution is 1:1000

Western Blot (WB) of anti-RAS antibody

The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS (AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.
Mouse Anti-RAS Monoclonal Antibody – Cat# MBS200206

Immunofluorescence (IF) of anti-RAS antibody

ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-RAS Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200206

Product References and Citations for anti-RAS antibody

Rocks O, et al. (2006). Current Opinion in Cell Biology 18 (4): 351-7.; Wennerberg K, et al. (March 2005). J. Cell. Sci. 118 (Pt 5): 843-6.; Rotblat B, et al. (2008). Methods Enzymol 439: 467-89.

Other Notes

Small volumes of anti-RAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAS purchase

MBS200206 is an antibody targeting RAS. Also known as RAS antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RAS. It may not necessarily be applicable to this product.

NCBI GI #
4505451
NCBI GeneID
4893
NCBI Accession #
NP_002515
UniProt Primary Accession #
P01111
UniProt Secondary Accession #
Q14971; Q15104; Q15282
UniProt Related Accession #
P01111
GenBank Nucleotide #
NM_002524.4
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
NRAS proto-oncogene, GTPase
NCBI Official Symbol
NRAS
NCBI Official Synonym Symbols
NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
UniProt Protein Name
GTPase NRas
UniProt Synonym Protein Names
Transforming protein N-Ras
Protein Family
Rosmarinate synthase
UniProt Gene Name
NRAS
UniProt Synonym Gene Names
HRAS1
NCBI Protein Information
GTPase NRas

NCBI Summary for RAS

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
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UniProt Comments for RAS

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Research Articles on RAS

1. NRAS was identified as a direct target of let-7e and promoted oncogenesis in glioma stem cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAS antibody

 Reference Product  PubMed Publications
 BRAF antibody  >1412 publications with RAS and BRAF
 EGFR antibody  >383 publications with RAS and EGFR
 PIK3CA antibody  >381 publications with RAS and PIK3CA
 ERBB2 antibody  >98 publications with RAS and ERBB2
 MAP2K1 antibody  >63 publications with RAS and MAP2K1
 RAF1 antibody  >38 publications with RAS and RAF1
 ERBB3 antibody  >11 publications with RAS and ERBB3
 ARAF antibody  >10 publications with RAS and ARAF
 PIK3CG antibody  >1 publications with RAS and PIK3CG

Pathways associated with anti-RAS antibody

 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of RAS In B Cells Pathway antibodies  Activation Of RAS In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Apelin Signaling Pathway antibodies  Apelin Signaling Pathway Diagram

Diseases associated with anti-RAS antibody

 Disease Name  Pubmed Publications
 Melanoma Antibodies  >864 publications with RAS and Melanoma
 Colorectal Neoplasms Antibodies  >343 publications with RAS and Colorectal Neoplasms
 Leukemia, Myeloid, Acute Antibodies  >202 publications with RAS and Leukemia, Myeloid, Acute
 Lung Neoplasms Antibodies  >157 publications with RAS and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >123 publications with RAS and Neoplasms, Experimental
 Lymphoma Antibodies  >107 publications with RAS and Lymphoma
 Neuroblastoma Antibodies  >105 publications with RAS and Neuroblastoma
 Cell Transformation, Neoplastic Antibodies  >102 publications with RAS and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >75 publications with RAS and Liver Neoplasms
 Thyroid cancer, follicular Antibodies  >71 publications with RAS and Thyroid cancer, follicular

Organs/Tissues associated with anti-RAS antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >472 publications with RAS and Skin
 Blood Antibodies  >273 publications with RAS and Blood
 Lung Antibodies  >210 publications with RAS and Lung
 Bone Antibodies  >139 publications with RAS and Bone
 Lymph Antibodies  >110 publications with RAS and Lymph
 Lymph Node Antibodies  >110 publications with RAS and Lymph Node
 Bone Marrow Antibodies  >104 publications with RAS and Bone Marrow
 Liver Antibodies  >103 publications with RAS and Liver
 Brain Antibodies  >79 publications with RAS and Brain
 Vascular Antibodies  >48 publications with RAS and Vascular
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