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Platelet Factor 4 (PF4) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002101
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MBS2002101 is an antibody targeting PF4. Also known as Polyclonal Antibody to Platelet Factor 4 (PF4). Reactive in Human. Host: Rabbit. More details

$195 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Platelet Factor 4 (PF4), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Platelet Factor 4 (PF4)
Product Gene Name
anti-PF4 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: PF4 (MBS2002101)
Biotin Conjugated Antibody: PF4 (MBS2003688)
Matching Pairs
Unconjugated Antibody: PF4 (MBS2002101)
Immunogen: PF4 (MBS2010107)
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OMIM
M25897 mRNA
3D Structure
ModBase 3D Structure for P02776
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant PF4 (Ala31~Ser101) expressed in E.coli (MBS2010107).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PF4 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-PF4 antibody
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-PF4 antibody

Western Blot: Sample: Recombinant PF4, Human.
Rabbit Anti-PF4 Polyclonal Antibody – Cat# MBS2002101

Immunohistochemistry (IHC) of anti-PF4 antibody

DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human PF4 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-PF4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002101

Immunohistochemistry (IHC) of anti-PF4 antibody

DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human PF4 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-PF4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002101

Other Notes

Small volumes of anti-PF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PF4. It may not necessarily be applicable to this product.

NCBI GI #
4505733
NCBI GeneID
5196
NCBI Accession #
NP_002610.1
UniProt Primary Accession #
P02776
UniProt Secondary Accession #
Q53X61; Q9UC64; Q9UC65
UniProt Related Accession #
P02776
GenBank Nucleotide #
NM_002619.3
NCBI Official Full Name
platelet factor 4
NCBI Official Synonym Full Names
platelet factor 4
NCBI Official Symbol
PF4
NCBI Official Synonym Symbols
PF-4; CXCL4; SCYB4
UniProt Protein Name
Platelet factor 4
UniProt Synonym Protein Names
C-X-C motif chemokine 4; Iroplact; Oncostatin-A
Protein Family
Platelet factor
UniProt Gene Name
PF4
UniProt Synonym Gene Names
CXCL4; SCYB4; PF-4
NCBI Protein Information
platelet factor 4

NCBI Summary for PF4

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]
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UniProt Comments for PF4

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Apoptosis; Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: chemokine activity; CXCR3 chemokine receptor binding; heparin binding; protein binding

Biological Process: cytokine-mediated signaling pathway; defense response to protozoan; G-protein coupled receptor signaling pathway; immune response; inflammatory response; killing by host of symbiont cells; leukocyte chemotaxis; negative regulation of angiogenesis; negative regulation of cytolysis; negative regulation of megakaryocyte differentiation; negative regulation of MHC class II biosynthetic process; platelet activation; platelet degranulation; positive regulation of cAMP metabolic process; positive regulation of macrophage differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; regulation of cell proliferation; regulation of megakaryocyte differentiation; response to lipopolysaccharide
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Research Articles on PF4

1. These findings support PF4 as a cancer-enhancing endocrine signal that controls discrete aspects of bone marrow hematopoiesis and tumor microenvironment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PF4 antibody

 Reference Product  PubMed Publications
 FGF2 antibody  >16 publications with PF4 and FGF2
 CXCR3 antibody  >14 publications with PF4 and CXCR3
 PROC antibody  >11 publications with PF4 and PROC
 CXCL11 antibody  >5 publications with PF4 and CXCL11

Pathways associated with anti-PF4 antibody

 Products by Pathway  Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram

Diseases associated with anti-PF4 antibody

 Disease Name  Pubmed Publications
 Thrombocytopenia Antibodies  >423 publications with PF4 and Thrombocytopenia
 Cardiovascular Diseases Antibodies  >352 publications with PF4 and Cardiovascular Diseases
 Thrombosis Antibodies  >286 publications with PF4 and Thrombosis
 Heart Diseases Antibodies  >117 publications with PF4 and Heart Diseases
 Neoplasms Antibodies  >96 publications with PF4 and Neoplasms
 Inflammation Antibodies  >70 publications with PF4 and Inflammation
 Nervous System Diseases Antibodies  >66 publications with PF4 and Nervous System Diseases
 Autoimmune Diseases Antibodies  >58 publications with PF4 and Autoimmune Diseases
 Kidney Diseases Antibodies  >54 publications with PF4 and Kidney Diseases
 Cerebral Infarction Antibodies  >35 publications with PF4 and Cerebral Infarction

Organs/Tissues associated with anti-PF4 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >92 publications with PF4 and Heart
 Bone Antibodies  >70 publications with PF4 and Bone
 Bone Marrow Antibodies  >61 publications with PF4 and Bone Marrow
 Skin Antibodies  >32 publications with PF4 and Skin
 Lung Antibodies  >31 publications with PF4 and Lung
 Embryonic Tissue Antibodies  >18 publications with PF4 and Embryonic Tissue
 Intestine Antibodies  >6 publications with PF4 and Intestine
 Placenta Antibodies  >1 publications with PF4 and Placenta
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