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Otubain 1 (OTUB1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002151
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2002151 is an antibody targeting OTUB1. Also known as Polyclonal Antibody to Otubain 1 (OTUB1). Reactive in Rat, Human, Mouse. Host: Rabbit. More details

$235 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Otubain 1 (OTUB1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Otubain 1 (OTUB1)
Product Gene Name
anti-OTUB1 antibody
Immunogen Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG S-MAAEEPQQ QK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHVFP EGSEPKVYLL YRPGHYDILY K
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat, Human, Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against OTUB1. It has been selected for its ability to recognize OTUB1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: OTUB1 (MBS2002151)
Biotin Conjugated Antibody: OTUB1 (MBS2001912)
Matching Pairs
Unconjugated Antibody: OTUB1 (MBS2002151)
Immunogen: OTUB1 (MBS2011421)
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Molecular Weight
31,270 Da
3D Structure
ModBase 3D Structure for B2RYG6
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant OTUB1 (Met1~Lys271) expressed in E.coli.
Cross Reactivity
Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083330
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-OTUB1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-OTUB1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-OTUB1 antibody

Western Blot: Sample: Rat Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat OTUB1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-OTUB1 Polyclonal Antibody – Cat# MBS2002151

Western Blot (WB) of anti-OTUB1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-OTUB1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002151

Immunohistochemistry (IHC) of anti-OTUB1 antibody

DAB staining on fromalin fixed paraffin- embedded stomach tissue)
Rabbit Anti-OTUB1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002151

Immunohistochemistry (IHC) of anti-OTUB1 antibody

DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Rabbit Anti-OTUB1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002151

Other Notes

Small volumes of anti-OTUB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OTUB1. It may not necessarily be applicable to this product.

NCBI GI #
157821731
NCBI GeneID
293705
NCBI Accession #
NP_001099802.1
UniProt Primary Accession #
B2RYG6
UniProt Related Accession #
B2RYG6
GenBank Nucleotide #
NM_001106332.1
NCBI Official Full Name
ubiquitin thioesterase OTUB1
NCBI Official Synonym Full Names
OTU deubiquitinase, ubiquitin aldehyde binding 1
NCBI Official Symbol
Otub1
UniProt Protein Name
Ubiquitin thioesterase OTUB1
UniProt Synonym Protein Names
Deubiquitinating enzyme OTUB1
Protein Family
Ubiquitin thioesterase
UniProt Gene Name
Otub1
NCBI Protein Information
ubiquitin thioesterase OTUB1

UniProt Comments for OTUB1

OTUB1: a protease that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occur when T-cell are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Two alternatively spliced isoforms are known.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; nucleus

Molecular Function: NEDD8-specific protease activity; thiol-dependent ubiquitin-specific protease activity; ubiquitin binding; ubiquitin protein ligase binding

Biological Process: adaptive immune response; cellular response to DNA damage stimulus; DNA repair
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Research Articles on OTUB1

1. OTUB1 may play a role in protecting the brain from secondary damage following intracerebral hemorrhage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-OTUB1 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >1 publications with OTUB1 and Kidney Diseases
 Acute Kidney Injury Antibodies  >1 publications with OTUB1 and Acute Kidney Injury
 Kidney Neoplasms Antibodies  >1 publications with OTUB1 and Kidney Neoplasms
 Disease Models, Animal Antibodies  >1 publications with OTUB1 and Disease Models, Animal

Organs/Tissues associated with anti-OTUB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >4 publications with OTUB1 and Kidney
 Ovary Antibodies  >1 publications with OTUB1 and Ovary
 Muscle Antibodies  >1 publications with OTUB1 and Muscle
 Brain Antibodies  >1 publications with OTUB1 and Brain
 Heart Antibodies  >1 publications with OTUB1 and Heart
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