• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Slit Homolog 1 (SLIT1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002160
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2002160 is an antibody targeting Slit1. Also known as Polyclonal Antibody to Slit Homolog 1 (Slit1). Reactive in Rat, Human, Mouse. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Slit Homolog 1 (Slit1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Slit Homolog 1 (Slit1)
Product Gene Name
anti-Slit1 antibody
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- LTPQRGSS SGLSRPELWL LLWAAAWRLG ATACPALCTC TGTTVDCHGT GLQAIPKNIP RNTERLELNG NNITRIHKND FAGLKQLRVL QLMENQIGAV ERGAFDDMKE LE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat, Human, Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against Slit1. It has been selected for its ability to recognize Slit1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: Slit1 (MBS2002160)
Biotin Conjugated Antibody: Slit1 (MBS2004865)
Matching Pairs
Unconjugated Antibody: Slit1 (MBS2002160)
Immunogen: Slit1 (MBS2012047)
Show more
Molecular Weight
164,638 Da
3D Structure
ModBase 3D Structure for O88279
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant Slit1 (Leu3~Glu112) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071136
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-Slit1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-Slit1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-Slit1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-SLIT1 Polyclonal Antibody – Cat# MBS2002160

Immunohistochemistry (IHC) of anti-Slit1 antibody

DAB staining on IHC-P; Samples: Rat Pancreas Tissue)
Rabbit Anti-SLIT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002160

Other Notes

Small volumes of anti-Slit1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Slit1. It may not necessarily be applicable to this product.

NCBI GI #
12621130
NCBI GeneID
65047
NCBI Accession #
NP_075242.1
UniProt Primary Accession #
O88279
UniProt Secondary Accession #
Q8CJG8; Q8CJG9; Q8CJH0; Q9QWL1; Q9WUG5
UniProt Related Accession #
O88279
GenBank Nucleotide #
NM_022953.2
NCBI Official Full Name
slit homolog 1 protein
NCBI Official Synonym Full Names
slit guidance ligand 1
NCBI Official Symbol
Slit1
NCBI Official Synonym Symbols
MEGF4
UniProt Protein Name
Slit homolog 1 protein
UniProt Synonym Protein Names
Multiple epidermal growth factor-like domains protein 4; Multiple EGF-like domains protein 4
Protein Family
Slit homolog 1 protein
UniProt Gene Name
Slit1
UniProt Synonym Gene Names
Megf4; Slit-1; Multiple EGF-like domains protein 4
NCBI Protein Information
slit homolog 1 protein

NCBI Summary for Slit1

homolog of Drosophila slit; may play a role in protein-protein interactions during midline formation in the central nervous system; specifically expressed in fetal and adult forebrain neurons [RGD, Feb 2006]

UniProt Comments for Slit1

SLIT1: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb (By similarity)

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q54

Cellular Component: extracellular space; intracellular

Molecular Function: calcium ion binding; heparan sulfate proteoglycan binding; protein binding; Roundabout binding

Biological Process: axon extension involved in axon guidance; axon guidance; axonogenesis; brain development; chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration; dorsal/ventral axon guidance; motor axon guidance; negative chemotaxis; negative regulation of axon extension involved in axon guidance; negative regulation of synaptogenesis; neuron projection morphogenesis; nuclear migration; olfactory bulb development; retinal ganglion cell axon guidance; spinal cord development; tangential migration from the subventricular zone to the olfactory bulb; telencephalon cell migration
Show more

Research Articles on Slit1

1. These findings indicate that Slit1/Robo2 signaling may have a role in regulating peripheral nerve regeneration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Slit1 antibody

 Reference Product  PubMed Publications
 Robo2 antibody  >65 publications with Slit1 and Robo2
 Robo1 antibody  >64 publications with Slit1 and Robo1
 Slit3 antibody  >52 publications with Slit1 and Slit3
 Dcc antibody  >14 publications with Slit1 and Dcc
 Robo4 antibody  >5 publications with Slit1 and Robo4
 Grem1 antibody  >2 publications with Slit1 and Grem1
 Ntn1 antibody  >2 publications with Slit1 and Ntn1
 Irx4 antibody  >1 publications with Slit1 and Irx4

Pathways associated with anti-Slit1 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-Slit1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >14 publications with Slit1 and Nervous System Diseases
 Abnormalities, Multiple Antibodies  >5 publications with Slit1 and Abnormalities, Multiple
 Breast Neoplasms Antibodies  >4 publications with Slit1 and Breast Neoplasms
 Nervous System Malformations Antibodies  >4 publications with Slit1 and Nervous System Malformations
 Adenocarcinoma Antibodies  >3 publications with Slit1 and Adenocarcinoma
 Liver Neoplasms Antibodies  >3 publications with Slit1 and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with Slit1 and Liver Diseases
 Inflammation Antibodies  >2 publications with Slit1 and Inflammation
 Heart Diseases Antibodies  >2 publications with Slit1 and Heart Diseases
 Bone Marrow Diseases Antibodies  >2 publications with Slit1 and Bone Marrow Diseases

Organs/Tissues associated with anti-Slit1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >89 publications with Slit1 and Brain
 Embryonic Tissue Antibodies  >52 publications with Slit1 and Embryonic Tissue
 Dorsal Root Ganglion Antibodies  >3 publications with Slit1 and Dorsal Root Ganglion
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000